Phage nanofibers induce vascularized osteogenesis in 3D printed bone scaffolds

Adv Mater. 2014 Aug 6;26(29):4961-4966. doi: 10.1002/adma.201400154. Epub 2014 Apr 7.

Abstract

A virus-activated matrix is developed to overcome the challenge of forming vascularized bone tissue. It is generated by filling a 3D printed bioceramic scaffold with phage nanofibers displaying high-density RGD peptide. After it is seeded with mesenchymal stem cells (MSCs) and implanted into a bone defect, the phage nanofibers induce osteogenesis and angiogenesis by activating endothelialization and osteogenic differentiation of MSCs.

Keywords: 3D printing; bone regeneration; nanofibers; phage display; scaffolds.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Bacteriophages / chemistry*
  • Biocompatible Materials / chemistry
  • Biocompatible Materials / pharmacology*
  • Bone and Bones / blood supply
  • Bone and Bones / cytology
  • Bone and Bones / drug effects
  • Bone and Bones / physiology*
  • Mesenchymal Stem Cells / drug effects
  • Nanofibers*
  • Neovascularization, Physiologic / drug effects*
  • Oligopeptides / chemistry
  • Osteogenesis / drug effects*
  • Printing, Three-Dimensional*
  • Tissue Scaffolds / chemistry

Substances

  • Biocompatible Materials
  • Oligopeptides
  • arginyl-glycyl-aspartic acid