Prevalence of asinine herpesvirus type 5 (AsHV-5) infection in clinically normal Lipizzaner horses

James Oliver Rushton; Jolanta Kolodziejek; Barbara Nell; Norbert Nowotny

doi:10.1016/j.tvjl.2013.10.035

Prevalence of asinine herpesvirus type 5 (AsHV-5) infection in clinically normal Lipizzaner horses

Vet J. 2014 Apr;200(1):200-3. doi: 10.1016/j.tvjl.2013.10.035. Epub 2013 Nov 9.

Authors

James Oliver Rushton¹, Jolanta Kolodziejek², Barbara Nell³, Norbert Nowotny⁴

Affiliations

¹ Viral Zoonoses, Emerging and Vector-Borne Infections Group, Institute of Virology, Department of Pathobiology, University of Veterinary Medicine Vienna, Veterinaerplatz 1, 1210 Vienna, Austria; Clinic for Surgery, Dentistry and Ophthalmology, Department of Companion Animals and Horses, University of Veterinary Medicine Vienna, Veterinaerplatz 1, 1210 Vienna, Austria.
² Viral Zoonoses, Emerging and Vector-Borne Infections Group, Institute of Virology, Department of Pathobiology, University of Veterinary Medicine Vienna, Veterinaerplatz 1, 1210 Vienna, Austria.
³ Clinic for Surgery, Dentistry and Ophthalmology, Department of Companion Animals and Horses, University of Veterinary Medicine Vienna, Veterinaerplatz 1, 1210 Vienna, Austria.
⁴ Viral Zoonoses, Emerging and Vector-Borne Infections Group, Institute of Virology, Department of Pathobiology, University of Veterinary Medicine Vienna, Veterinaerplatz 1, 1210 Vienna, Austria; Department of Microbiology and Immunology, College of Medicine and Health Sciences, Sultan Qaboos University, Muscat, Oman. Electronic address: Norbert.Nowotny@vetmeduni.ac.at.

PMID: 24709517
DOI: 10.1016/j.tvjl.2013.10.035

Abstract

The aim of this study was to assess the extent of asinine herpesvirus (AsHV) type 5 infection in 'closed' populations of clinically normal Lipizzaner horses. Peripheral blood mononuclear cells plus nasal and conjunctival swabs were obtained on four occasions over an 18 month period from 266 animals as part of a health surveillance programme. Sequence analysis of samples that were positive by nested consensus herpesvirus PCR but negative using quantified equid herpesvirus (EHV) type 2 and 5 PCR, revealed a total of 51 samples from 39 horses positive for AsHV-5. No statistically significant association between animal age, gender or geographical location and infection status was identified. The findings suggest sub-clinical AsHV-5 infection may be encountered more frequently than previously reported.

Keywords: Asinine herpesvirus (AsHV) type 5; Equid gammaherpesviruses; Horse; Lipizzaner.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Austria / epidemiology
Base Sequence
Herpesviridae / genetics*
Herpesviridae / isolation & purification
Herpesviridae Infections / epidemiology
Herpesviridae Infections / veterinary*
Herpesviridae Infections / virology
Horse Diseases / epidemiology*
Horse Diseases / virology
Horses
Leukocytes, Mononuclear / virology
Molecular Sequence Data
Nose / virology
Polymerase Chain Reaction / veterinary
Prevalence
Sequence Alignment / veterinary
Viral Proteins / genetics*
Viral Proteins / metabolism

Substances

Viral Proteins

Associated data

GENBANK/KC825357