Engineering cells to improve protein expression

Su Xiao; Joseph Shiloach; Michael J Betenbaugh

doi:10.1016/j.sbi.2014.03.005

Engineering cells to improve protein expression

Curr Opin Struct Biol. 2014 Jun:26:32-8. doi: 10.1016/j.sbi.2014.03.005. Epub 2014 Apr 3.

Authors

Su Xiao¹, Joseph Shiloach², Michael J Betenbaugh³

Affiliations

¹ Biotechnology Core Laboratory, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA; Department of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, MD 21218, USA.
² Biotechnology Core Laboratory, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, MD 20892, USA.
³ Department of Chemical and Biomolecular Engineering, Johns Hopkins University, Baltimore, MD 21218, USA. Electronic address: beten@jhu.edu.

Abstract

Cellular engineering of bacteria, fungi, insect cells and mammalian cells is a promising methodology to improve recombinant protein production for structural, biochemical, and commercial applications. Increased understanding of the host organism biology has suggested engineering strategies targeting bottlenecks in transcription, translation, protein processing and secretory pathways, as well as cell growth and survival. A combination of metabolic engineering and synthetic biology has been used to improve the properties of cells for protein production, which has resulted in enhanced yields of multiple protein classes.

Publication types

Research Support, N.I.H., Extramural
Review

MeSH terms

Animals
Cell Engineering / methods*
Gene Expression
Humans
Mutagenesis
Protein Folding
Recombinant Proteins / biosynthesis
Recombinant Proteins / chemistry
Recombinant Proteins / genetics*
Transcription, Genetic

Substances

Recombinant Proteins

Abstract

Publication types

MeSH terms

Substances

Grants and funding