Graphite-like carbon nitrides as peroxidase mimetics and their applications to glucose detection

Tianran Lin; Liangshuang Zhong; Jing Wang; Liangqia Guo; Hanyin Wu; Qingquan Guo; FengFu Fu; Guonan Chen

doi:10.1016/j.bios.2014.03.023

Graphite-like carbon nitrides as peroxidase mimetics and their applications to glucose detection

Biosens Bioelectron. 2014 Sep 15:59:89-93. doi: 10.1016/j.bios.2014.03.023. Epub 2014 Mar 26.

Authors

Tianran Lin¹, Liangshuang Zhong¹, Jing Wang¹, Liangqia Guo², Hanyin Wu¹, Qingquan Guo¹, FengFu Fu³, Guonan Chen¹

Affiliations

¹ Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, and Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350108, China.
² Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, and Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350108, China. Electronic address: lqguo@fzu.edu.cn.
³ Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, and Department of Chemistry, Fuzhou University, Fuzhou, Fujian 350108, China. Electronic address: fengfu@fzu.edu.cn.

PMID: 24704762
DOI: 10.1016/j.bios.2014.03.023

Abstract

g-C3N4 was found to possess intrinsic peroxidase-like activity, and could catalytically oxidize 3,3',5,5'-tetramethylbenzidine (TMB) by H2O2 to produce a color reaction. Using g-C3N4 peroxidase-like catalytic activity and glucose oxidase (GOx), a colorimetric method for glucose detection in serum samples has been developed. The linear range for glucose was from 5 to 100 μM (R(2)=0.9987) and the limit of detection was as low as 1.0 μM. The proposed method was applied to detect glucose in serum samples by the naked eyes.

Keywords: Carbon nitride; Glucose; Peroxidase; Sensor; Superconductor.

Publication types

Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Biomimetic Materials / chemistry*
Biosensing Techniques / methods*
Blood Glucose / analysis*
Colorimetry / methods
Graphite / chemistry*
Humans
Hydrogen Peroxide / analysis
Limit of Detection
Nitriles / chemistry*
Oxidation-Reduction
Peroxidase / chemistry*

Substances

Blood Glucose
Nitriles
cyanogen
Graphite
Hydrogen Peroxide
Peroxidase