A new method for yeast phagocytosis analysis by flow cytometry

Catarina Carneiro; Catarina Vaz; Joana Carvalho-Pereira; Célia Pais; Paula Sampaio

doi:10.1016/j.mimet.2014.03.013

A new method for yeast phagocytosis analysis by flow cytometry

J Microbiol Methods. 2014 Jun:101:56-62. doi: 10.1016/j.mimet.2014.03.013. Epub 2014 Apr 3.

Authors

Catarina Carneiro¹, Catarina Vaz¹, Joana Carvalho-Pereira¹, Célia Pais¹, Paula Sampaio²

Affiliations

¹ Centre of Molecular and Environmental Biology (CBMA), Department of Biology, University of Minho, Braga, Portugal.
² Centre of Molecular and Environmental Biology (CBMA), Department of Biology, University of Minho, Braga, Portugal. Electronic address: psampaio@bio.uminho.pt.

PMID: 24704495
DOI: 10.1016/j.mimet.2014.03.013

Abstract

Herein we developed a method based on the quenching effect of propidium iodide over Sytox-Green fluorescence to assess yeast phagocytosis by flow cytometry. It allows accurate quantification of living from dead phagocytes; internalized from non-internalized cells, maintaining yeast fluorescence within phagocytes; and the different associations between phagocytes and fungal cells.

Keywords: Candida spp; Flow cytometry; PAMPs; Phagocytosis; Propidium iodide; Sytox Green.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Candida / cytology
Candida / isolation & purification*
Cell Line
Flow Cytometry / methods*
Macrophages / physiology
Mice
Organic Chemicals
Phagocytosis / physiology*
Propidium

Substances

Organic Chemicals
SYTOX Green
Propidium