Identification of multiple diagnostic SNP loci for differentiation of three salmonid species using SNP-arrays

Agata Drywa; Anita Poćwierz-Kotus; Stefan Dobosz; Matthew P Kent; Sigbjørn Lien; Roman Wenne

doi:10.1016/j.margen.2014.03.003

Identification of multiple diagnostic SNP loci for differentiation of three salmonid species using SNP-arrays

Mar Genomics. 2014 Jun:15:5-6. doi: 10.1016/j.margen.2014.03.003. Epub 2014 Apr 2.

Authors

Agata Drywa¹, Anita Poćwierz-Kotus², Stefan Dobosz³, Matthew P Kent⁴, Sigbjørn Lien⁴, Roman Wenne⁵

Affiliations

¹ National Marine Fisheries Research Institute, 81-332 Gdynia, Poland.
² Institute of Oceanology PAS, 81-712 Sopot, Poland.
³ Inland Fisheries Institute, 10-719 Olsztyn, Poland.
⁴ Centre for Integrative Genetics (CIGENE), Department of Animal and Aquacultural Sciences, Norwegian University of Life Sciences, N-1432 Ås, Norway.
⁵ Institute of Oceanology PAS, 81-712 Sopot, Poland. Electronic address: rwenne@iopan.gda.pl.

PMID: 24703883
DOI: 10.1016/j.margen.2014.03.003

Abstract

This paper reports the use of SNP-array technology in a cross-species study for non-ambiguous species identifications. Based on an existing SNP-array for Atlantic salmon (cross)hybridisations with samples of salmon, brown trout and rainbow trout were analyzed to identify species-specific diagnostic markers. In total 566 SNP loci were identified to be highly polymorphic across the three salmonid species providing the molecular basement for various monitoring applications in aquaculture and food industries.

Keywords: Genotyping; Oncorhynchus; SNP array; Salmo; Salmonids.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Analysis of Variance
Animals
Aquaculture
Genetic Markers / genetics*
Genotype
Polymorphism, Single Nucleotide / genetics*
Salmonidae / genetics*
Species Specificity

Substances

Genetic Markers