N-Cadherin and Tie2 positive CD34⁺CD38⁻CD123⁺ leukemic stem cell populations can develop acute myeloid leukemia more effectively in NOD/SCID mice

Leuk Res. 2014 May;38(5):632-7. doi: 10.1016/j.leukres.2014.03.007. Epub 2014 Mar 19.

Abstract

Emerging studies suggest that the population of malignant cells found in human acute myelogenous leukemia (AML) arises from a rare population of leukemic stem cells (LSCs). A lot of investigators have reported the identification of cell surface markers, such as CD123. Here, we report the identification of N-cadherin and Tie2 as LSCs markers. Inoculation of CD34(+)CD38(-)CD123(+)N-cadherin(+) and CD34(+)CD38(-)CD123(+) Tie2(+) population can induce leukemia in NOD/SCID mice. The leukemic blast cells from the primary leukemic mice could also induce leukemia in the secondary transplantation. These findings suggested that N-cadherin and Tie2 were the important markers that can assist in leukemia development.

Keywords: Acute myeloid leukemia; Leukemic stem cell; N-Cadherin; Tie2.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • ADP-ribosyl Cyclase 1 / analysis*
  • Adolescent
  • Adult
  • Aged
  • Animals
  • Antigens, CD34 / analysis*
  • Cadherins / analysis*
  • Female
  • Humans
  • Interleukin-3 Receptor alpha Subunit / analysis*
  • Leukemia, Myeloid, Acute / etiology*
  • Leukemia, Myeloid, Acute / pathology
  • Male
  • Mice
  • Mice, Inbred NOD
  • Mice, SCID
  • Middle Aged
  • Neoplastic Stem Cells / chemistry
  • Neoplastic Stem Cells / cytology*
  • Receptor, TIE-2 / analysis*

Substances

  • Antigens, CD34
  • Cadherins
  • IL3RA protein, human
  • Interleukin-3 Receptor alpha Subunit
  • Receptor, TIE-2
  • ADP-ribosyl Cyclase 1