Inhibition of the electron transport strongly affects transcription and transcript levels in Arabidopsis mitochondria

Yan O Zubo; Tatyana V Potapova; Maria V Yamburenko; Vladislav I Tarasenko; Yuri M Konstantinov; Thomas Börner

doi:10.1016/j.mito.2014.03.011

Inhibition of the electron transport strongly affects transcription and transcript levels in Arabidopsis mitochondria

Mitochondrion. 2014 Nov:19 Pt B:222-30. doi: 10.1016/j.mito.2014.03.011. Epub 2014 Mar 31.

Authors

Yan O Zubo¹, Tatyana V Potapova², Maria V Yamburenko¹, Vladislav I Tarasenko³, Yuri M Konstantinov⁴, Thomas Börner⁵

Affiliations

¹ Institute of Biology-Genetics, Humboldt University, Chaussestr. 117, 10115 Berlin, Germany.
² Institute of Biology-Genetics, Humboldt University, Chaussestr. 117, 10115 Berlin, Germany; The Siberian Institute of Plant Physiology and Biochemistry SB RAS, Lermontova St., 132, Irkutsk 664033, Russia.
³ The Siberian Institute of Plant Physiology and Biochemistry SB RAS, Lermontova St., 132, Irkutsk 664033, Russia.
⁴ Institute of Biology-Genetics, Humboldt University, Chaussestr. 117, 10115 Berlin, Germany; The Siberian Institute of Plant Physiology and Biochemistry SB RAS, Lermontova St., 132, Irkutsk 664033, Russia; The Irkutsk State University, Sukhe-Batar St., 5, Irkutsk 664033, Russia.
⁵ Institute of Biology-Genetics, Humboldt University, Chaussestr. 117, 10115 Berlin, Germany. Electronic address: thomas.boerner@rz.hu-berlin.de.

PMID: 24699356
DOI: 10.1016/j.mito.2014.03.011

Abstract

Mitochondrial transcription rate and RNA steady-state levels were examined in shoots of Arabidopsis seedlings. The shoots were treated with inhibitors of complex III and IV of the cytochrome pathway (CP) and with an inhibitor of the alternative oxidase (AOX) of the mitochondrial electron transport chain. The inhibition of AOX and CP complexes III and IV affected transcription and transcript levels in different ways. CP and AOX inhibitors had opposite effects. Our data support the idea that the redox state of the electron transport chain is involved in the regulation of mitochondrial gene expression at transcriptional and post-transcriptional levels.

Keywords: Alternative oxidase; Electron transport chain; Mitochondrial RNA; Mitochondrial gene expression; Redox regulation; Retrograde signaling.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Arabidopsis / drug effects*
Arabidopsis / metabolism*
Electron Transport / drug effects*
Enzyme Inhibitors / metabolism
Mitochondria / drug effects*
Mitochondria / metabolism*
Oxidation-Reduction
Plant Shoots / drug effects
Plant Shoots / metabolism
RNA / metabolism
RNA, Messenger / biosynthesis*
RNA, Mitochondrial
Seedlings / drug effects
Seedlings / metabolism
Transcription, Genetic / drug effects*

Substances

Enzyme Inhibitors
RNA, Messenger
RNA, Mitochondrial
RNA