Context: Solid lipid nanoparticles (SLNs) can efficiently and efficaciously incorporate anti-cancer agents.
Objective: To prepare and characterise tamoxifen (TAM)-loaded SLNs.
Materials and methods: Glyceryl monostearate, Tween-80, and trehalose were used in SLNs. SLNs were tested via dynamic light scattering (DLS), transmission electron microscopy (TEM), differential scanning calorimetry (DSC), X-ray diffraction (XRD), and Fourier transform infrared spectroscopy (FTIR).
Results: Characterisation studies revealed SLNs of about 540 nm with a negative surface charge and confirmed the entrapment of TAM in the SLNs. The entrapment efficiency was estimated to be 60%.
Discussion: The in vitro drug release profile demonstrated a gradual increase followed by a release plateau for several days. A drug concentration-dependent increase in cytotoxic activity was observed when the SLNs were evaluated in cell cultures.
Conclusion: Biocompatible and stable lyophilised SLNs were successfully prepared and found to possess properties that may be utilised in an anti-cancer drug delivery system.
Keywords: Biocompatibility; FTIR; X-ray powder diffractometry; dynamic light scattering; solid lipid nanoparticles; zeta potential.