Abstract
micro RNAs (miRNAs) are small non-coding RNAs that act as posttranscriptional repressors by binding to the 3'-UTR of target mRNAs. On the other hand, mesenchymal-epithelial transition (EMT) and kidney fibrosis is a pathological process of chronic kidney disease (CKD), and its relationship to miRNAs is becoming recognized as a potential target for CKD therapies. To find new miRNAs involved in EMT, we examined miRNA expression in experimental models of EMT and renal epithelialization using microarray, and found that miR-34c attenuates EMT induced by TGF-β in a mouse tubular cell line. To confirm the effects of miR-34c in vivo, we administered the precursor of miR-34c to mice with unilateral ureteral obstruction, and miR-34c decreased kidney fibrosis area and the expression of connective tissue growth factor, α-SMA, collagen type 1, collagen type 3 and fibronectin. In conclusion, our study showed miR-34c attenuates EMT and kidney fibrosis of mice with ureteral obstruction.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Actins / genetics
-
Actins / metabolism
-
Animals
-
Calcium-Binding Proteins / metabolism
-
Cell Line
-
Collagen Type I / genetics
-
Collagen Type I / metabolism
-
Collagen Type III / genetics
-
Collagen Type III / metabolism
-
Connective Tissue Growth Factor / genetics
-
Connective Tissue Growth Factor / metabolism
-
Epithelial-Mesenchymal Transition* / drug effects
-
Fibronectins / genetics
-
Fibronectins / metabolism
-
Fibrosis
-
Intercellular Signaling Peptides and Proteins / metabolism
-
Kidney / metabolism
-
Kidney / pathology
-
Membrane Proteins / metabolism
-
Mice
-
MicroRNAs / antagonists & inhibitors
-
MicroRNAs / genetics
-
MicroRNAs / metabolism*
-
Oligonucleotides, Antisense / metabolism
-
Serrate-Jagged Proteins
-
Transforming Growth Factor beta / pharmacology
-
Ureteral Obstruction / genetics
-
Ureteral Obstruction / pathology*
Substances
-
Acta2 protein, mouse
-
Actins
-
Calcium-Binding Proteins
-
Collagen Type I
-
Collagen Type III
-
Fibronectins
-
Intercellular Signaling Peptides and Proteins
-
Membrane Proteins
-
MicroRNAs
-
Oligonucleotides, Antisense
-
Serrate-Jagged Proteins
-
Transforming Growth Factor beta
-
Connective Tissue Growth Factor