Saccharomyces cerevisiae Rif1 cooperates with MRX-Sae2 in promoting DNA-end resection

EMBO Rep. 2014 Jun;15(6):695-704. doi: 10.1002/embr.201338338. Epub 2014 Apr 1.

Abstract

Diverse roles in DNA metabolism have been envisaged for budding yeast and mammalian Rif1. In particular, yeast Rif1 is involved in telomere homeostasis, while its mammalian counterpart participates in the cellular response to DNA double-strand breaks (DSBs). Here, we show that Saccharomyces cerevisiae Rif1 supports cell survival to DNA lesions in the absence of MRX or Sae2. Furthermore, it contributes to the nucleolytic processing (resection) of DSBs. This Rif1-dependent control of DSB resection becomes important for DSB repair by homologous recombination when resection activities are suboptimal.

Keywords: Rad9; Rif1; Saccharomyces cerevisiae; double‐strand break; resection.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chromatin Immunoprecipitation
  • DNA Breaks, Double-Stranded*
  • DNA Repair / physiology*
  • DNA-Binding Proteins / metabolism
  • Electrophoresis, Agar Gel
  • Endodeoxyribonucleases / metabolism
  • Endonucleases / metabolism*
  • Exodeoxyribonucleases / metabolism
  • Multiprotein Complexes / metabolism*
  • Repressor Proteins / metabolism*
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae / metabolism
  • Saccharomyces cerevisiae Proteins / metabolism*
  • Telomere-Binding Proteins / metabolism*

Substances

  • DNA-Binding Proteins
  • Multiprotein Complexes
  • RAD50 protein, S cerevisiae
  • Repressor Proteins
  • SAE2 protein, S cerevisiae
  • Saccharomyces cerevisiae Proteins
  • Telomere-Binding Proteins
  • XRS2 protein, S cerevisiae
  • RIF1 protein, S cerevisiae
  • Endodeoxyribonucleases
  • Endonucleases
  • Exodeoxyribonucleases
  • MRE11 protein, S cerevisiae
  • exodeoxyribonuclease I