A reverse transcription loop-mediated isothermal amplification assay to rapidly diagnose foot-and-mouth disease virus C

J Vet Sci. 2014;15(3):423-6. doi: 10.4142/jvs.2014.15.3.423. Epub 2014 Apr 2.

Abstract

A reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed to rapidly detect foot-and-mouth disease virus serotype C (FMDV C). By testing 10-fold serial dilutions of FMDV C samples, sensitivity of the FMDV C RT-LAMP was found to be 10 times higher than that of conventional reverse transcription- PCR (RT-PCR). No cross-reactivity with A, Asia 1, or O FMDV or swine vesicular disease virus (SVDV) indicated that FMDV C RT-LAMP may be an exciting novel method for detecting FMDV C.

Keywords: foot-and-mouth disease; foot-and-mouth disease virus serotype C; reverse transcription loop-mediated isothermal amplification; reverse transcription-PCR; sensitivity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Foot-and-Mouth Disease / diagnosis*
  • Foot-and-Mouth Disease Virus / genetics
  • Nucleic Acid Amplification Techniques / methods*
  • Nucleic Acid Amplification Techniques / veterinary
  • Reverse Transcriptase Polymerase Chain Reaction / veterinary
  • Reverse Transcription / genetics
  • Sensitivity and Specificity