Neurotoxic effects of indocyanine green -cerebellar granule cell culture viability study

Beata Toczylowska; Elzbieta Zieminska; Grazyna Goch; Daniel Milej; Anna Gerega; Adam Liebert

doi:10.1364/BOE.5.000800

Neurotoxic effects of indocyanine green -cerebellar granule cell culture viability study

Biomed Opt Express. 2014 Feb 19;5(3):800-16. doi: 10.1364/BOE.5.000800. eCollection 2014 Mar 1.

Authors

Beata Toczylowska¹, Elzbieta Zieminska², Grazyna Goch³, Daniel Milej⁴, Anna Gerega⁴, Adam Liebert⁴

Affiliations

¹ Nalecz Institute of Biocybernetics and Biomedical Engineering, Polish Academy of Sciences, 4 Trojdena Str. 02-109 Warsaw, Poland ; Institute of Biochemistry and Biophysics, Polish Academy of Sciences, 5A Pawinskiego Str., 02-106 Warsaw, Poland.
² Mossakowski Medical Research Centre, Polish Academy of Sciences, 5 Pawinskiego Str., 02-106 Warsaw, Poland.
³ Institute of Biochemistry and Biophysics, Polish Academy of Sciences, 5A Pawinskiego Str., 02-106 Warsaw, Poland.
⁴ Nalecz Institute of Biocybernetics and Biomedical Engineering, Polish Academy of Sciences, 4 Trojdena Str. 02-109 Warsaw, Poland.

Abstract

The aim of this study was to examine neurotoxicity indocyanine green (ICG). We assessed viability of primary cerebellar granule cell culture (CGC) exposed to ICG to test two mechanisms that could be the first triggers causing neuronal toxicity: imbalance in calcium homeostasis and the degree of oligomerization of ICG molecules. We have observed this imbalance in CGC after exposure to 75-125μΜ ICG and dose and application sequence dependent protective effect of Gadovist on surviving neurons in vitro when used with ICG. Spectroscopic studies suggest the major cause of toxicity of the ICG is connected with oligomers formation. ICG at concentration of 25 μM (which is about 4 times higher than the highest concentration of ICG in the brain applied in in-vivo human studies) is not neurotoxic in the cell culture.

Keywords: (170.0170) Medical optics and biotechnology; (170.1530) Cell analysis; (170.1610) Clinical applications; (170.6280) Spectroscopy, fluorescence and luminescence.