Choosing between RT-qPCR and RNA-seq: a back-of-the-envelope estimate towards the definition of the break-even-point

Anal Bioanal Chem. 2014 Jun;406(15):3533-6. doi: 10.1007/s00216-014-7687-x.

Authors

Alberto Nonis¹, Barbara De Nardi, Alessandro Nonis

Affiliation

¹ Department of Agronomy, Food, Natural Resources, Animals and Environment, University of Padua, Viale dell'Università 16, 35020, Legnaro, PD, Italy, alberto.nonis@unipd.it.

PMID: 24687434
DOI: 10.1007/s00216-014-7687-x

No abstract available

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Gene Expression Profiling
High-Throughput Nucleotide Sequencing
Humans
Oligonucleotide Array Sequence Analysis
RNA / genetics*
Real-Time Polymerase Chain Reaction / methods*
Reverse Transcriptase Polymerase Chain Reaction / economics
Reverse Transcriptase Polymerase Chain Reaction / methods*
Sequence Analysis, RNA / economics
Sequence Analysis, RNA / methods*
Software

Substances

RNA