Binuclear copper complexes: synthesis, X-ray structure and interaction study with nucleotide/protein by in vitro biochemical and electrochemical analysis

M Alagesan; N S P Bhuvanesh; N Dharmaraj

doi:10.1016/j.ejmech.2014.03.043

Binuclear copper complexes: synthesis, X-ray structure and interaction study with nucleotide/protein by in vitro biochemical and electrochemical analysis

Eur J Med Chem. 2014 May 6:78:281-93. doi: 10.1016/j.ejmech.2014.03.043. Epub 2014 Mar 19.

Authors

M Alagesan¹, N S P Bhuvanesh², N Dharmaraj³

Affiliations

¹ Inorganic and Nanomaterials Research Laboratory, Department of Chemistry, Bharathiar University, Coimbatore 641 046, India.
² Department of Chemistry, Texas A&M University, College Station, TX 77843, USA.
³ Inorganic and Nanomaterials Research Laboratory, Department of Chemistry, Bharathiar University, Coimbatore 641 046, India. Electronic address: dharmaraj@buc.edu.in.

PMID: 24686015
DOI: 10.1016/j.ejmech.2014.03.043

Abstract

Two new, binuclear copper(II) hydrazone complexes have been synthesized and characterized by various physico-chemical techniques including single crystal X-ray diffraction. Interaction of these complexes with nucleotide and protein were analyzed by in vitro biochemical and electrochemical analysis. Both the complexes exhibited intercalative mode of binding with DNA. Further, gel electrophoresis assay demonstrated the ability of the complexes to cleave the supercoiled pBR322 plasmid DNA to nicked circular DNA form. Cytotoxicity of the complexes performed against a panel of cancer cell lines and a normal cell line proved that these complexes are potentially cytotoxic against the cancerous cell lines, particularly with IC50 as low as 0.7 μM against HeLa cell line.

Keywords: Binuclear copper complex; Cytotoxicity; Nucleotide and protein interaction.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Antineoplastic Agents / chemical synthesis
Antineoplastic Agents / chemistry
Antineoplastic Agents / pharmacology*
Cattle
Cell Line, Tumor
Cell Proliferation / drug effects
Copper / chemistry*
Crystallography, X-Ray
DNA / chemistry
DNA / drug effects*
Dose-Response Relationship, Drug
Drug Screening Assays, Antitumor
Electrochemical Techniques*
HeLa Cells
Humans
Models, Molecular
Molecular Structure
Organometallic Compounds / chemical synthesis
Organometallic Compounds / chemistry
Organometallic Compounds / pharmacology*
Serum Albumin / chemistry*
Structure-Activity Relationship

Substances

Antineoplastic Agents
Organometallic Compounds
Serum Albumin
Copper
DNA