Niabella thaonhiensis sp. nov., isolated from the forest soil of Kyonggi University in Korea

Curr Microbiol. 2014 Aug;69(2):176-81. doi: 10.1007/s00284-014-0565-0. Epub 2014 Mar 30.

Abstract

Strain NHI-24(T) was isolated from forest soil by a polycarbonate membrane transwell plate. It is a Gram-negative, rod-shaped, non-motile, non-spore-forming bacterium. Phylogenetic analysis based on 16S rRNA gene sequence data indicated that strain NHI-24(T) is closely related to members of the genus Niabella: N. drilacis E90(T) (97.7 %), N. tibetensis 15-4(T) (96.7 %), N. aurantiaca R2A15-11(T) (96.5 %), N. hirudinis E96(T) (95.8 %), N. soli JS13-8(T) (94.7 %), N. ginsengisoli NBRC106414(T) (94.4 %), and N. yanshanensis CCBAU 05354(T) (94.2 %). Growth temperatures range widely, from 15 to 37 °C, with 30 °C as the optimum. Salt tolerance ranges from 0 to 2 %. The strain grows at pH 6.5-11.0, with an optimal range of pH 7.0-9.5. Cells produce flexirubin-type pigments, and the predominant menaquinone is MK-7. The major fatty acids of strain NHI-24(T) are iso-C15:0 (36.72 %), iso-C15:1 G (20.8 %), and summed feature 3 (C16:1 ω7c/C16:1 ω6c; 15.2 %). DNA-DNA hybridization values between strain NHI-24(T) and members of the genus Niabella range from 37 to 53 %. Based on these results, it is proposed that strain NHI-24(T) represents a novel species of the genus Niabella with the name Niabella thaonhiensis (= KACC 17215(T) = KEMB 9005-018(T) = JCM 18864(T)).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacteroidetes / classification*
  • Bacteroidetes / genetics
  • Bacteroidetes / isolation & purification*
  • Bacteroidetes / physiology
  • Cluster Analysis
  • Cytosol / chemistry
  • DNA, Bacterial / chemistry
  • DNA, Bacterial / genetics
  • DNA, Ribosomal / chemistry
  • DNA, Ribosomal / genetics
  • Fatty Acids / analysis
  • Forests
  • Hydrogen-Ion Concentration
  • Korea
  • Molecular Sequence Data
  • Nucleic Acid Hybridization
  • Phylogeny
  • Pigments, Biological
  • RNA, Ribosomal, 16S / genetics
  • Sequence Analysis, DNA
  • Soil Microbiology*
  • Temperature
  • Universities
  • Vitamin K 2 / analysis

Substances

  • DNA, Bacterial
  • DNA, Ribosomal
  • Fatty Acids
  • Pigments, Biological
  • RNA, Ribosomal, 16S
  • Vitamin K 2

Associated data

  • GENBANK/JQ796720