Two-signal electrochemical method for evaluation suppression and proliferation of MCF-7 cells based on intracellular purine

Anal Biochem. 2014 Jul 1:456:1-5. doi: 10.1016/j.ab.2014.03.013. Epub 2014 Mar 28.

Abstract

Two electrochemical signals ascribed to xanthine/guanine and hypanthine/adenine in MCF-7 cells were detected at 0.726 and 1.053 V, respectively. Based on the intensity of signals, the genistein-induced proliferation and suppression of MCF-7 cells could be evaluated. The results showed that with the increase of genistein dose at the range of 10(-9) to 10(-6)M, the two electrochemical signals of MCF-7 cell suspension increased due to the proliferation, whereas the tendency at the high dosage range of more than 10(-5)M was decreased. The proliferation and cytotoxicity obtained by the electrochemical method were in agreement with those obtained by cell counting and the MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium] method. Thus, the two-signal electrochemical method is an effective way to evaluate the effect of drugs on cell activity based on purine metabolism.

Keywords: Cell activity; Electrochemistry; Genistein; MCF-7 cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antineoplastic Agents / pharmacology*
  • Cell Proliferation / drug effects
  • Dose-Response Relationship, Drug
  • Drug Screening Assays, Antitumor / instrumentation
  • Drug Screening Assays, Antitumor / methods*
  • Electrochemistry / instrumentation
  • Electrochemistry / methods*
  • Electrodes
  • Genistein / pharmacology*
  • Humans
  • Intracellular Space / drug effects
  • Intracellular Space / metabolism*
  • MCF-7 Cells
  • Nanotubes, Carbon / chemistry
  • Purines / chemistry
  • Purines / metabolism*

Substances

  • Antineoplastic Agents
  • Nanotubes, Carbon
  • Purines
  • Genistein
  • purine