Identification and analysis of differential miRNAs in PK-15 cells after foot-and-mouth disease virus infection

PLoS One. 2014 Mar 27;9(3):e90865. doi: 10.1371/journal.pone.0090865. eCollection 2014.

Abstract

The alterations of MicroRNAs(miRNAs) in host cell after foot-and-mouth disease virus (FMDV) infection is still obscure. To increase our understanding of the pathogenesis of FMDV at the post-transcriptional regulation level, Solexa high-throu MicroRNAs (miRNAs) play an important role both in the post-transcriptional regulation of gene expression and host-virus interactions. Despite investigations of miRNA expression ghput sequencing and bioinformatic tools were used to identify differentially expressed miRNAs and analyze their functions during FMDV infection of PK-15 cells. Results indicated that 9,165,674 and 9,230,378 clean reads were obtained, with 172 known and 72 novel miRNAs differently expressed in infected and uninfected groups respectively. Some of differently expressed miRNAs were validated using stem-loop real-time quantitative RT-PCR. The GO annotation and KEGG pathway analysis for target genes revealed that differently expressed miRNAs were involved in immune response and cell death pathways.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Line
  • Computational Biology / methods
  • Foot-and-Mouth Disease / genetics
  • Foot-and-Mouth Disease / virology
  • Foot-and-Mouth Disease Virus / physiology*
  • Gene Expression Profiling
  • Gene Expression Regulation
  • Gene Library
  • Gene Ontology
  • High-Throughput Nucleotide Sequencing
  • MicroRNAs / genetics*
  • Molecular Sequence Annotation
  • Reproducibility of Results

Substances

  • MicroRNAs

Grants and funding

This work was supported by The National Natural Science Foundation of China (Grant No. 31201914), China Postdoctoral Science Foundation (2013M530683), and China Agriculture Research System (CARS-39). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.