Using ion exchange chromatography to purify a recombinantly expressed protein

Abstract

Ion exchange chromatography (IEX) separates molecules by their surface charge, a property that can vary vastly between different proteins. There are two types of IEX, cation exhange and anion exchange chromatography. The protocol that follows was designed by the authors for anion exchange chromatography of a recombinantly expressed protein having a pI of 4.9 and containing two cysteine residues and one tryptophan residue, using an FPLC system. Prior to anion exchange, the protein had been salted out using ammonium sulfate precipitation and partially purified via hydrophobic interaction chromatography (see Salting out of proteins using ammonium sulfate precipitation and Use and Application of Hydrophobic Interaction Chromatography for Protein Purification). Slight modifications to this protocol may be made to accommodate both the protein of interest and the availability of equipment.

Keywords: Carboxymethyl (CM); Diethylaminoethyl (DEAE); FPLC system; Ion exchange chromatography (IEX); Quaternary aminoethyl (QAE); Quaternary anion (Q); Sulfopropyl (SP).