The two step nanotube formation on TiZr as scaffolds for cell growth

Sabina Grigorescu; Vasile Pruna; Irina Titorencu; Victor V Jinga; Anca Mazare; Patrik Schmuki; Ioana Demetrescu

doi:10.1016/j.bioelechem.2014.03.002

The two step nanotube formation on TiZr as scaffolds for cell growth

Bioelectrochemistry. 2014 Aug:98:39-45. doi: 10.1016/j.bioelechem.2014.03.002. Epub 2014 Mar 10.

Authors

Sabina Grigorescu¹, Vasile Pruna², Irina Titorencu², Victor V Jinga², Anca Mazare³, Patrik Schmuki⁴, Ioana Demetrescu¹

Affiliations

¹ University Politehnica Bucharest, Faculty of Applied Chemistry and Materials Science, Department of General Chemistry, 1-7 Polizu, Bucharest 011061, Romania.
² Institute of Cellular Biology and Pathology "Nicolae Simionescu" of the Romanian Academy, Romania.
³ University of Erlangen-Nürnberg, Department of Materials Science, LKO, Martensstraße 7, 91058 Erlangen, Germany.
⁴ University of Erlangen-Nürnberg, Department of Materials Science, LKO, Martensstraße 7, 91058 Erlangen, Germany; King Abdulaziz University, Chemistry Department, Jeddah, Saudi Arabia.

PMID: 24662040
DOI: 10.1016/j.bioelechem.2014.03.002

Abstract

Various TiO2 nanotubes on Ti50Zr alloy have been fabricated via a two step anodization method in glycol with 15vol.% H2O and 0.2M NH4F under anodization controlled voltages of 15, 30 and 45V. A new sonication treatment in deionized water with three steps and total sonication time as 1min was performed after the first anodization step in order to remove the oxide layer grown during 2h. The second step of anodization was for 1h and took place at the same conditions. The role of removed layer as a nano-prepatterned surface was evidenced in the formation of highly ordered nanotubular structures and morphological features were analyzed by SEM, AFM and surface wettability. The voltage-controlled anodization leads to various nanoarhitectures, with diameters in between 20 and 80nm. As biological assay, cell culture tests with MG63 cell line originally derived from a human osteosarcoma were performed. A correlation between nanostructure morphological properties as a result of voltage-controlled anodization and cell response was established.

Keywords: Anodization; Cell response; TiO(2) nanotubes; TiZr alloy.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Actins / genetics
Alloys / chemistry*
Biocompatible Materials / chemistry*
Cell Adhesion / physiology
Cell Culture Techniques
Cell Line, Tumor
Cell Proliferation / physiology*
Cell Survival / physiology
Gene Expression
Humans
Microscopy, Atomic Force
Microscopy, Electron, Scanning
Nanotechnology / methods*
Nanotubes / chemistry*
Osteoblasts / drug effects
Osteoblasts / metabolism
Osteocalcin / genetics
Osteonectin / genetics
Particle Size
Surface Properties
Titanium / chemistry
Wettability
Zinc / chemistry

Substances

Actins
Alloys
Biocompatible Materials
Osteonectin
Osteocalcin
titanium dioxide
Titanium
Zinc