Anti-inflammatory effects of extract from Haliotis discus hannai fermented with Cordyceps militaris mycelia in RAW264.7 macrophages through TRIF-dependent signaling pathway

Fish Shellfish Immunol. 2014 May;38(1):184-9. doi: 10.1016/j.fsi.2014.03.018. Epub 2014 Mar 20.

Abstract

In this study, Haliotis discus hannai (H. discus hannai) fermentation was attempted with Cordyceps militaris (C. militaris) mycelia using a solid culture. We tried to ferment H. discus hannai to determine the optimal conditions fermentation with regards to its anti-inflammatory effects. The extracts of H. discus hannai fermented with C. militaris mycelia (HFCM-5) showed higher nitric oxide inhibitory effects than H. discus hannai and C. militaris alone. HFCM-5 also decreased pro-inflammatory cytokines, TNF-α and IL-6 in a dose-dependent manner. HFCM-5 did not affect the MyD88-dependent pathway, but decreased phosphorylation of IRF3 and STAT1 which are involved in TRIF-dependent pathway. Taken together, our results suggest that HFCM-5 exerts its anti-inflammatory effects via TRIF signaling pathway and could potentially be used as a functional food in the regulation of inflammation.

Keywords: Anti-inflammatory; Cordyceps militaris; Fermentation; Haliotis discus hannai.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adaptor Proteins, Vesicular Transport / physiology*
  • Animals
  • Anti-Inflammatory Agents / pharmacology*
  • Cell Extracts / pharmacology*
  • Cell Survival
  • Cordyceps*
  • Cytokines / genetics
  • Cytokines / metabolism
  • Fermentation
  • Gene Expression Regulation / drug effects
  • Macrophages / physiology*
  • Mice
  • Mollusca / chemistry*
  • Mycelium / physiology
  • Signal Transduction

Substances

  • Adaptor Proteins, Vesicular Transport
  • Anti-Inflammatory Agents
  • Cell Extracts
  • Cytokines