A case-control study evaluating RT-PCR/ESI-MS technology compared to direct fluorescent antibody and xTAG RVP PCR

Diagn Microbiol Infect Dis. 2014 Jun;79(2):187-9. doi: 10.1016/j.diagmicrobio.2014.02.009. Epub 2014 Feb 24.

Abstract

Waste nasopharyngeal swabs (N = 244) were evaluated by the reverse-transcriptase polymerase chain reaction/electrospray ionization mass spectrometry PLEX-ID Broad Respiratory Virus Surveillance Kit version 2.5 compared to direct fluorescent antibody and xTAG Respiratory Virus Panel for percent agreement, sensitivity, and specificity. Sensitivity and specificity were 91% (111/122) and 95.1% (116/122), respectively. Sensitivity by virus, except parainfluenza, was 92.9-100%, and specificity was 99-100%.

Keywords: DFA; RT-PCR/ESI-MS; Respiratory virus diagnostics; xTAG RVP.

Publication types

  • Comparative Study
  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Case-Control Studies
  • Fluorescent Antibody Technique, Direct / methods
  • Humans
  • Polymerase Chain Reaction / methods*
  • Respiratory Tract Infections / diagnosis*
  • Respiratory Tract Infections / virology
  • Sensitivity and Specificity
  • Spectrometry, Mass, Electrospray Ionization / methods*
  • Virus Diseases / diagnosis*
  • Virus Diseases / virology