One-pot green synthesis of carbon dots by using Saccharum officinarum juice for fluorescent imaging of bacteria (Escherichia coli) and yeast (Saccharomyces cerevisiae) cells

Mater Sci Eng C Mater Biol Appl. 2014 May 1:38:20-7. doi: 10.1016/j.msec.2014.01.038. Epub 2014 Jan 29.

Abstract

We are reporting highly economical plant-based hydrothermal method for one-pot green synthesis of water-dispersible fluorescent carbon dots (CDs) by using Saccharum officinarum juice as precursor. The synthesized CDs were characterized by UV-visible, fluorescence, Fourier transform infrared (FT-IR), dynamic light scattering (DLS), high-resolution transmission electron microscopic (HR-TEM), and laser scanning confocal microscopic techniques. The CDs are well dispersed in water with an average size of ~3 nm and showed bright blue fluorescence under UV-light (λex=365 nm). These CDs acted as excellent fluorescent probes in cellular imaging of bacteria (Escherichia coli) and yeast (Saccharomyces cerevisiae).

Keywords: Bacteria and yeast cells; DLS; Fluorescence microscopy; Saccharum officinarum juice; TEM.

MeSH terms

  • Beverages*
  • Carbon / chemistry*
  • Escherichia coli / cytology*
  • Green Chemistry Technology / methods*
  • Microbial Viability
  • Microscopy, Electron, Transmission
  • Saccharomyces cerevisiae / cytology*
  • Saccharum / chemistry*
  • Spectrometry, Fluorescence
  • Spectrophotometry, Ultraviolet
  • Spectroscopy, Fourier Transform Infrared
  • Temperature
  • X-Ray Diffraction

Substances

  • Carbon