A novel system for cloning human TCRs: Cutting short the way to TCR-based anticancer therapy

Eiji Kobayashi; Hiroyuki Kishi; Atsushi Muraguchi

doi:10.4161/onci.27258

A novel system for cloning human TCRs: Cutting short the way to TCR-based anticancer therapy

Oncoimmunology. 2014 Jan 1;3(1):e27258. doi: 10.4161/onci.27258.

Authors

Eiji Kobayashi¹, Hiroyuki Kishi¹, Atsushi Muraguchi¹

Affiliation

¹ Department of Immunology; Graduate School of Medicine and Pharmaceutical Sciences; University of Toyama; Toyama, Japan.

Abstract

T-cell receptor (TCR)-based gene immunotherapy has emerged as a promising approach for the treatment of multiple malignancies. We have recently reported an efficient system for the cloning and functional evaluation of TCR-coding cDNAs. This system, which we named hTEC10, allows for the determination of TCR antigen specificity in less than 10 days, and may therefore constitute a fast and powerful platform for the development of new TCR-based anticancer therapies.

Keywords: T-cell receptor; gene therapy; hTEC10; single-cell RT-PCR.