Abstract
We investigated the effect of auxin and acetylcholine on the expression of the tomato expansin gene LeEXPA2, a specific expansin gene expressed in elongating tomato hypocotyl segments. Since auxin interferes with clathrin-mediated endocytosis, in order to regulate cellular and developmental responses we produced protoplasts from tomato elongating hypocotyls and followed the endocytotic marker, FM4-64, internalization in response to treatments. Tomato protoplasts were observed during auxin and acetylcholine treatments after transient expression of chimerical markers of volume-control related compartments such as vacuoles. Here we describe the contribution of auxin and acetylcholine to LeEXPA2 expression regulation and we support the hypothesis that a possible subcellular target of acetylcholine signal is the vesicular transport, shedding some light on the characterization of this small molecule as local mediator in the plant physiological response.
MeSH terms
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2,4-Dichlorophenoxyacetic Acid / pharmacology
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Acetylcholine / pharmacology*
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Cell Size / drug effects*
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Endocytosis / drug effects
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Endoplasmic Reticulum / metabolism
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Gene Expression Regulation, Plant / drug effects
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Hypocotyl / cytology
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Hypocotyl / drug effects
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Hypocotyl / genetics
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Indoleacetic Acids / pharmacology
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Luminescent Proteins / genetics
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Luminescent Proteins / metabolism
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Microscopy, Confocal
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Plant Cells / drug effects*
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Plant Cells / metabolism
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Plant Growth Regulators / pharmacology
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Plant Proteins / genetics
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Protein Transport / drug effects
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Protoplasts / cytology
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Protoplasts / drug effects
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Protoplasts / metabolism
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Pyridinium Compounds / metabolism
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Quaternary Ammonium Compounds / metabolism
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Reverse Transcriptase Polymerase Chain Reaction
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Solanum lycopersicum / cytology
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Solanum lycopersicum / drug effects*
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Solanum lycopersicum / genetics
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Vacuoles / metabolism
Substances
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FM 4-64
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Indoleacetic Acids
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Luminescent Proteins
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Plant Growth Regulators
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Plant Proteins
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Pyridinium Compounds
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Quaternary Ammonium Compounds
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expansin protein, plant
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2,4-Dichlorophenoxyacetic Acid
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Acetylcholine