[Production of the monoclonal antibodies to the rabies virus nucleoprotein]

Vopr Virusol. 2013 Sep-Oct;58(5):38-43.
[Article in Russian]

Abstract

Five hybridomas secreting monoclonal antibodies (MAbs) for the nucleocapsid protein of the rabies virus were obtained through the fusion of the SP2/0 murine myeloma cells with splenocytes of BALB/c mice immunized with fixed rabies virus (CVS strain). All hybridomas secret MAbs of the IgG class that display different specificity to the nucleocapsids of rabies and rabies-related viruses. MAbs 2ell showed the specificity for the prevalent in Russia rabies viruses that are similar to commercially available anti-rabies conjugate.

Publication types

  • English Abstract

MeSH terms

  • Animals
  • Antibodies, Monoclonal / biosynthesis*
  • Antibodies, Monoclonal / immunology
  • Antibody Specificity
  • Cats
  • Dogs
  • Foxes
  • Humans
  • Hybridomas
  • Immunization
  • Mice
  • Mice, Inbred BALB C
  • Mustelidae
  • Nucleocapsid Proteins / genetics
  • Nucleocapsid Proteins / immunology*
  • Rabies / diagnosis
  • Rabies / virology
  • Rabies virus / genetics
  • Rabies virus / immunology*
  • Rabies virus / isolation & purification
  • Russia
  • Wolves

Substances

  • Antibodies, Monoclonal
  • Nucleocapsid Proteins