Abstract
In the bivoltine strain of the silkworm, Bombyx mori, embryonic diapause is induced transgenerationally as a maternal effect. Progeny diapause is determined by the environmental temperature during embryonic development of the mother; however, its molecular mechanisms are largely unknown. Here, we show that the Bombyx TRPA1 ortholog (BmTrpA1) acts as a thermosensitive transient receptor potential (TRP) channel that is activated at temperatures above ∼ 21 °C and affects the induction of diapause in progeny. In addition, we show that embryonic RNAi of BmTrpA1 affects diapause hormone release during pupal-adult development. This study identifying a thermosensitive TRP channel that acts as a molecular switch for a relatively long-term predictive adaptive response by inducing an alternative phenotype to seasonal polyphenism is unique.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Body Weight
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Bombyx / embryology*
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Bombyx / genetics
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Bombyx / metabolism*
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Diapause, Insect / genetics*
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Embryo, Nonmammalian / metabolism*
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Embryonic Development / genetics
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Gene Expression Profiling
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Gene Expression Regulation, Developmental
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Gene Knockdown Techniques
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HEK293 Cells
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Humans
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Inheritance Patterns / genetics*
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Insect Proteins / genetics
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Insect Proteins / metabolism*
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Ion Channel Gating
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Molecular Sequence Data
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Neuroendocrine Cells / cytology
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Neuroendocrine Cells / metabolism
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Neuropeptides / metabolism
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Phenotype
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Pupa / cytology
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Pupa / metabolism
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RNA Interference
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TRPC Cation Channels / genetics
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TRPC Cation Channels / metabolism*
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Temperature*
Substances
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Insect Proteins
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Neuropeptides
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TRPC Cation Channels
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diapause hormone
Associated data
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GENBANK/AB243445
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GENBANK/AB264789
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GENBANK/AB437368
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GENBANK/AB437369
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GENBANK/AB703646
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GENBANK/AB703647