Abstract
Protein isolates of pumpkin (Cucurbita pepo L) seeds were hydrolyzed by acid protease to prepare antioxidative peptides. The hydrolysis conditions were optimized through Box-Behnken experimental design combined with response surface method (RSM). The second-order model, developed for the DPPH radical scavenging activity of pumpkin seed hydrolysates, showed good fit with the experiment data with a high value of coefficient of determination (0.9918). The optimal hydrolysis conditions were determined as follows: hydrolyzing temperature 50°C, pH 2.5, enzyme amount 6000 U/g, substrate concentration 0.05 g/ml and hydrolyzing time 5 h. Under the above conditions, the scavenging activity of DPPH radical was as high as 92.82%.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Analysis of Variance
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Antioxidants / metabolism*
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Biotechnology / methods*
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Biphenyl Compounds / metabolism
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Cucurbita / enzymology
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Cucurbita / metabolism*
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Free Radical Scavengers / pharmacology
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Hydrolysis / drug effects
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Models, Biological
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Peptides / isolation & purification*
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Picrates / metabolism
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Regression Analysis
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Reproducibility of Results
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Seeds / enzymology
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Seeds / metabolism*
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Substrate Specificity / drug effects
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Time Factors
Substances
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Antioxidants
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Biphenyl Compounds
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Free Radical Scavengers
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Peptides
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Picrates
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1,1-diphenyl-2-picrylhydrazyl
Grants and funding
This work was supported by the Natural Science Foundation of Shanxi (2012011031-4). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.