Optimization of preparation of antioxidative peptides from pumpkin seeds using response surface method

PLoS One. 2014 Mar 17;9(3):e92335. doi: 10.1371/journal.pone.0092335. eCollection 2014.

Abstract

Protein isolates of pumpkin (Cucurbita pepo L) seeds were hydrolyzed by acid protease to prepare antioxidative peptides. The hydrolysis conditions were optimized through Box-Behnken experimental design combined with response surface method (RSM). The second-order model, developed for the DPPH radical scavenging activity of pumpkin seed hydrolysates, showed good fit with the experiment data with a high value of coefficient of determination (0.9918). The optimal hydrolysis conditions were determined as follows: hydrolyzing temperature 50°C, pH 2.5, enzyme amount 6000 U/g, substrate concentration 0.05 g/ml and hydrolyzing time 5 h. Under the above conditions, the scavenging activity of DPPH radical was as high as 92.82%.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Analysis of Variance
  • Antioxidants / metabolism*
  • Biotechnology / methods*
  • Biphenyl Compounds / metabolism
  • Cucurbita / enzymology
  • Cucurbita / metabolism*
  • Free Radical Scavengers / pharmacology
  • Hydrolysis / drug effects
  • Models, Biological
  • Peptides / isolation & purification*
  • Picrates / metabolism
  • Regression Analysis
  • Reproducibility of Results
  • Seeds / enzymology
  • Seeds / metabolism*
  • Substrate Specificity / drug effects
  • Time Factors

Substances

  • Antioxidants
  • Biphenyl Compounds
  • Free Radical Scavengers
  • Peptides
  • Picrates
  • 1,1-diphenyl-2-picrylhydrazyl

Grants and funding

This work was supported by the Natural Science Foundation of Shanxi (2012011031-4). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.