Cytotoxicity and anti-inflammatory effects of root bark extracts of Acanthopanax henryi

Chin J Nat Med. 2014 Feb;12(2):121-5. doi: 10.1016/S1875-5364(14)60019-4.

Abstract

Aim: To investigate the cytotoxicity, anti-inflammatory activity, and action mechanism of root bark extracts of Acanthopanax henryi.

Method: The hot methanol extract of the root bark of A. henryi was subjected to XAD-4 column chromatography eluting with a gradient of methanol in water. The cytotoxicity and anti-inflammatory effects of the MeOH fractions were evaluated on the inhibition on lipopolysaccharide (LPS)-induced nitric oxide, prostaglandin E2, interleukin-1β, and interleukin-6 production in RAW 264.7 macrophages.

Results: The 80% MeOH fraction was a better inhibitor of LPS-induced NO, PGE2, IL-1β, and IL-6 production, and expression of inducible nitric oxide synthase (iNOS) at the protein levels in a concentration-dependent manner.

Conclusion: The 80% MeOH fraction of A. henryi root bark has significant anti-inflammatory activity. This provides a pharmacological basis for clinical application for the treatment of inflammation.

Keywords: Acanthopanax henryi (Oliv.) Harms; Anti-inflammatory activity; Cytotoxic activity; Root bark extract.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Anti-Inflammatory Agents / pharmacology
  • Anti-Inflammatory Agents / therapeutic use*
  • Dinoprostone / metabolism
  • Dose-Response Relationship, Drug
  • Eleutherococcus*
  • Inflammation / chemically induced
  • Inflammation / drug therapy*
  • Inflammation / metabolism
  • Interleukin-1beta / metabolism
  • Interleukin-6 / metabolism
  • Lipopolysaccharides
  • Macrophages / drug effects*
  • Macrophages / metabolism
  • Mice
  • Nitric Oxide / metabolism
  • Nitric Oxide Synthase Type II / metabolism
  • Phytotherapy*
  • Plant Bark
  • Plant Extracts / pharmacology
  • Plant Extracts / therapeutic use*
  • Plant Roots

Substances

  • Anti-Inflammatory Agents
  • Interleukin-1beta
  • Interleukin-6
  • Lipopolysaccharides
  • Plant Extracts
  • Nitric Oxide
  • Nitric Oxide Synthase Type II
  • Dinoprostone