Developmental validation of a forensic rapid DNA-STR kit: Expressmarker 16

Huaigu Zhou; Dan Wu; Ronghua Chen; Yan Xu; Zifang Xia; Yulin Guo; Fan Zhang; Weiguo Zheng

doi:10.1016/j.fsigen.2014.02.008

Developmental validation of a forensic rapid DNA-STR kit: Expressmarker 16

Forensic Sci Int Genet. 2014 Jul:11:31-8. doi: 10.1016/j.fsigen.2014.02.008. Epub 2014 Feb 23.

Authors

Huaigu Zhou¹, Dan Wu², Ronghua Chen², Yan Xu², Zifang Xia³, Yulin Guo³, Fan Zhang³, Weiguo Zheng³

Affiliations

¹ Institute of Forensic Science, Shanghai Municipal Public Security Bureau/Key Laboratory of Forensic Evidence and Scene Technology, Ministry of Public Security/Shanghai Key Laboratory of Crime Scene Evidence, Shanghai 200083, China. Electronic address: hgzhou803@hotmail.com.
² Institute of Forensic Science, Shanghai Municipal Public Security Bureau/Key Laboratory of Forensic Evidence and Scene Technology, Ministry of Public Security/Shanghai Key Laboratory of Crime Scene Evidence, Shanghai 200083, China.
³ AGCU ScienTech Incorporation, Wuxi 214174, China.

PMID: 24631694
DOI: 10.1016/j.fsigen.2014.02.008

Abstract

DNA-STR analysis is widely used in the forensic science field and has important requirements on the analysis time to obtain faster inspections. The developed forensic STR kit, referred to as Expressmarker 16 (EX16), could shorten the amplification time to a minimum of 35min. It enables 16 STR loci to be co-detected, including 13 CODIS loci, D2S1338, D6S1043 and Amelogenin loci. The kit is validated by a series of tests formed by DNA mixtures, stutter ratios, optimized PCR protocols based on annealing temperature research, species specificities, inhibitors, sensitivity, and parallel tests according to FBI QAS (2009/2011) (QAS, 2009; SWGDAM, 2010). The results demonstrated that EX16 was a useful tool for rapid criminal investigation.

Keywords: Forensic DNA analysis; Rapid identification; STR.

Publication types

Validation Study

MeSH terms

Animals
DNA / genetics*
Forensic Genetics*
Genetic Markers*
Humans
Microsatellite Repeats / genetics*
Polymerase Chain Reaction
Species Specificity

Substances

Genetic Markers
DNA