Differentiation of Klebsiella pneumoniae carbapenemase (KPC) variants by pyrosequencing

J Microbiol Methods. 2014 May:100:42-5. doi: 10.1016/j.mimet.2014.02.013. Epub 2014 Mar 11.

Abstract

A fast and reliable protocol using the pyrosequencing technique was developed to identify 11 different types of the KPC enzyme. A total of 65 blaKPC positive bacterial isolates were tested and characterized. In the end, the pyrosequencing proved to be a powerful tool for epidemiological studies of KPC producer isolates.

Keywords: KPC; Klebsiella pneumoniae carbapenemase; Pyrosequencing; Real-time PCR.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Proteins / classification*
  • Bacterial Proteins / genetics*
  • Genetic Variation*
  • Humans
  • Klebsiella Infections / epidemiology
  • Klebsiella Infections / microbiology
  • Klebsiella pneumoniae / classification
  • Klebsiella pneumoniae / enzymology*
  • Klebsiella pneumoniae / genetics
  • Molecular Epidemiology / methods
  • Sequence Analysis, DNA / methods*
  • beta-Lactamases / classification*
  • beta-Lactamases / genetics*

Substances

  • Bacterial Proteins
  • beta-Lactamases
  • carbapenemase