Abstract
Arabidopsis GDSL lipase 1 (GLIP1) has been shown to modulate systemic immunity through the regulation of ethylene signaling components. Here we demonstrate that the constitutive triple response mutant ctr1-1 requires GLIP1 for the ethylene response, gene expression, and pathogen resistance. The glip1-1 mutant was defective in induced resistance following primary inoculation of necrotrophic pathogens, whereas GLIP1-overexpressing plants showed resistance to multiple pathogens. Necrotrophic infection triggered the downregulation of EIN3 and the activation of ERF1 and SID2 in a GLIP1-dependent manner. These results suggest that GLIP1 positively and negatively regulates ethylene signaling, resulting in an ethylene-associated, necrotroph-induced immune response.
Keywords:
EIN3; ERF1; Ethylene; GLIP1; Salicylic acid; Systemic immunity.
Copyright © 2014 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Arabidopsis / enzymology*
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Arabidopsis / immunology
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Arabidopsis / microbiology
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Arabidopsis Proteins / genetics
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Arabidopsis Proteins / metabolism
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Arabidopsis Proteins / physiology*
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Base Sequence
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Carboxylic Ester Hydrolases / physiology*
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DNA-Binding Proteins
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Disease Resistance*
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Ethylenes / metabolism*
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Gene Expression
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Gene Expression Regulation, Plant / immunology
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Host-Pathogen Interactions
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Intramolecular Transferases / metabolism
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Nuclear Proteins / genetics
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Nuclear Proteins / metabolism
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Peptide Termination Factors / genetics
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Peptide Termination Factors / metabolism
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Plant Diseases / immunology
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Plant Diseases / microbiology
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Plant Growth Regulators / metabolism
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Plant Leaves / enzymology
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Plant Leaves / immunology
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Plant Leaves / microbiology
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Pseudomonas syringae / physiology
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Sequence Analysis, DNA
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Signal Transduction
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Transcription Factors / genetics
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Transcription Factors / metabolism
Substances
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Arabidopsis Proteins
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DNA-Binding Proteins
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EIN3 protein, Arabidopsis
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Ethylenes
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Nuclear Proteins
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Peptide Termination Factors
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Plant Growth Regulators
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Transcription Factors
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eukaryotic release factor 1, Arabidopsis
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ethylene
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Carboxylic Ester Hydrolases
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Glip1 protein, Arabidopsis
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Intramolecular Transferases
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isochorismate synthase