Differential miRNA expression defines migration and reduced apoptosis in follicular thyroid carcinomas

Mol Cell Endocrinol. 2014 May 5;388(1-2):1-9. doi: 10.1016/j.mce.2014.02.011. Epub 2014 Mar 12.

Abstract

The objective of the study was to identify microRNAs (miRs) characteristic for follicular thyroid carcinoma (FTC) and to define their role in tumorigenesis. A miR-microarray study was conducted to identify miRs differentially expressed between FTCs and their surrounding tissues. Selection was further reinforced by a literature review. Four miRs were selected and confirmed by RT-qPCR: miR-146b, -183, -221 were up-regulated, whereas miR-199b down-regulated in FTCs. The influence of these miRs on cell proliferation, cell cycle, apoptosis and migration was studied in HTori and FTC-133 cells. Functional characterization suggests an impact of miR-183 and miR-146b in FTC development. Overexpression of both miRs significantly induces migration. Moreover, overexpression of miR-183 significantly represses apoptosis. MiR-199b and -221 do not have significant effects on proliferation, cell cycle, apoptosis or migration in HTori and FTC-133 cells. Our data suggest that miR-146b and miR-183 may influence FTC development through the induction of migration and apoptosis inhibition.

Keywords: Apoptosis; Follicular thyroid cancer; Migration; miRNA.

Publication types

  • Review

MeSH terms

  • Adenocarcinoma, Follicular / genetics
  • Adenocarcinoma, Follicular / metabolism*
  • Adenocarcinoma, Follicular / pathology
  • Apoptosis*
  • Cell Line, Tumor
  • Cell Movement*
  • Cell Proliferation
  • Gene Expression Regulation, Neoplastic
  • Humans
  • MicroRNAs / genetics
  • MicroRNAs / metabolism*
  • Thyroid Neoplasms / genetics
  • Thyroid Neoplasms / metabolism*
  • Thyroid Neoplasms / pathology

Substances

  • MIRN146 microRNA, human
  • MIRN183 microRNA, human
  • MicroRNAs