Antioxidant activity of bovine casein hydrolysates produced by Ficus carica L.-derived proteinase

Giovanna Di Pierro; Martina B O'Keeffe; Alexey Poyarkov; Giovanna Lomolino; Richard J FitzGerald

doi:10.1016/j.foodchem.2014.01.080

Antioxidant activity of bovine casein hydrolysates produced by Ficus carica L.-derived proteinase

Food Chem. 2014 Aug 1:156:305-11. doi: 10.1016/j.foodchem.2014.01.080. Epub 2014 Feb 5.

Authors

Giovanna Di Pierro¹, Martina B O'Keeffe², Alexey Poyarkov³, Giovanna Lomolino⁴, Richard J FitzGerald⁵

Affiliations

¹ Department of Life Sciences, University of Limerick, Limerick, Ireland; Department of Agronomy, Food, Natural Resources, Animals and Environment - DAFNAE, Università di Padova, Legnaro, Padova, Italy.
² Department of Life Sciences, University of Limerick, Limerick, Ireland.
³ Department of Life Sciences, University of Limerick, Limerick, Ireland; Food for Health Ireland, Department of Life Sciences, University of Limerick, Limerick, Ireland.
⁴ Department of Agronomy, Food, Natural Resources, Animals and Environment - DAFNAE, Università di Padova, Legnaro, Padova, Italy.
⁵ Department of Life Sciences, University of Limerick, Limerick, Ireland; Food for Health Ireland, Department of Life Sciences, University of Limerick, Limerick, Ireland. Electronic address: dick.fitzgerald@ul.ie.

PMID: 24629973
DOI: 10.1016/j.foodchem.2014.01.080

Abstract

A Ficus carica L. latex proteinase preparation was investigated for its ability to produce antioxidant hydrolysates/peptides from bovine casein (CN). The Oxygen Radical Absorbance Capacity (ORAC) values for NaCN and β-CN hydrolysates ranged from 0.06 to 0.18, and from 0.51 to 1.19μmol Trolox equivalents/mg freeze-dried sample, respectively. Gel permeation HPLC showed that the β-CN hydrolysate with a degree of hydrolysis of 21% had 65% of peptide material with a molecular mass <500Da. The RP-UPLC profiles also indicated that β-CN was substantially hydrolysed during the early stages of hydrolysis. Analysis of the 4h β-CN hydrolysate by LC-ESI-MS/MS allowed identification of 8 peptide sequences with potential antioxidant properties.

Keywords: Antioxidant activity; Bioactive peptides; Degree of hydrolysis; Ficus carica L.; Mass spectrometry; ORAC(FL) assay; Sodium caseinate; β-Casein.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Antioxidants / chemistry
Caseins / chemistry*
Cattle
Chromatography, High Pressure Liquid / methods*
Chromatography, Liquid / methods*
Ficus / chemistry*
Oxidation-Reduction
Tandem Mass Spectrometry / methods*

Substances

Antioxidants
Caseins
casein hydrolysate