A novel photodynamic therapy for drug-resistant prostate cancer cells using porphyrus envelope as a novel photosensitizer

Masaya Yamauchi; Norihiro Honda; Hisanao Hazama; Shoji Tachikawa; Hiroyuki Nakamura; Yasufumi Kaneda; Kunio Awazu

doi:10.1016/j.pdpdt.2013.10.001

A novel photodynamic therapy for drug-resistant prostate cancer cells using porphyrus envelope as a novel photosensitizer

Photodiagnosis Photodyn Ther. 2014 Mar;11(1):48-54. doi: 10.1016/j.pdpdt.2013.10.001. Epub 2013 Oct 19.

Authors

Masaya Yamauchi¹, Norihiro Honda², Hisanao Hazama³, Shoji Tachikawa⁴, Hiroyuki Nakamura⁴, Yasufumi Kaneda⁵, Kunio Awazu⁶

Affiliations

¹ Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan. Electronic address: yamauchi-m@see.eng.osaka-u.ac.jp.
² Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan; Institute for Academic Initiatives, Osaka University, 1-1 Yamadaoka, Suita, Osaka 565-0871, Japan.
³ Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan.
⁴ Faculty of Science, Gakushuin University, 1-5-1 Mejiro, Toshima-ku, Tokyo 171-8588, Japan.
⁵ Graduate School of Medicine, Osaka University, 2-2 Yamadaoka, Suita, Osaka 565-0871, Japan.
⁶ Graduate School of Engineering, Osaka University, 2-1 Yamadaoka, Suita, Osaka 565-0871, Japan; Graduate School of Frontier Biosciences, Osaka University, 1-3 Yamadaoka, Suita, Osaka 565-0871, Japan; The Center for Advanced Medical Engineering and Informatics, Osaka University, 2-2 Yamadaoka, Suita, Osaka 565-0871, Japan.

PMID: 24629697
DOI: 10.1016/j.pdpdt.2013.10.001

Abstract

Background: In the clinic, it is often very difficult to treat drug-resistant advanced prostate cancer by conventional treatments. Photodynamic therapy (PDT) is a minimally invasive treatment that takes advantage of photochemical reactions between a photosensitizer and light. On the basis of several of its key characteristics, PDT is considered to be a promising novel method for treating drug-resistant prostate cancer.

Objectives: For effective treatment of drug-resistant prostate cancer, we developed a novel agent termed porphyrus envelope, which was produced from PpIX lipid and hemagglutinating virus of Japan envelope (HVJ-E).

Materials and methods: We inserted PpIX lipid into HVJ-E by centrifugation, and used the resultant porphyrus envelope in PDT of two drug-resistant prostate cancer cell lines, PC-3 and PC-3-DR.

Results: Porphyrus envelope enhanced uptake of PpIX, and cytotoxicity of PDT, relative to free PpIX lipid or PpIX induced by 5-ALA.

Conclusion: PDT using porphyrus envelope has potential as a method for treating drug-resistant prostate cancer.

Keywords: Drug delivery system; Drug resistance; Hemagglutinating virus of Japan envelope; Photodynamic therapy; Prostate cancer.

MeSH terms

Aminolevulinic Acid / administration & dosage
Aminolevulinic Acid / therapeutic use*
Cell Line, Tumor
Drug Delivery Systems
Humans
Male
Photochemotherapy / methods*
Photosensitizing Agents / administration & dosage
Photosensitizing Agents / therapeutic use*
Prostatic Neoplasms / drug therapy*
Protoporphyrins
Sendai virus

Substances

Photosensitizing Agents
Protoporphyrins
Aminolevulinic Acid
protoporphyrin IX