Abstract
The aim of this study was to investigate the effect of Quercus mongolica (QM) which induce anti-photoaging process of skin in vitro. Bioassay-guided isolation of 80 % Me2CO extract of the leaves of QM led to the isolation and identification of six known phenolic compounds: pedunculagin (1), (-)-epigallocatechin (2), (+)-catechin (3), quercetin 3-O-(6″-O-galloyl)-β-D-glucopyranoside (4), kaempferol-3-O-β-D-glucopyranoside-7-O-α-L-rhamnopyranoside (5) and kaempferol 3-O-(6″-galloyl)-β-D-glucopyranoside (6). The effects of compounds 1-6 on expression of matrix metalloproteinase-1 (MMP-1) and type-I procollagen were further evaluated. Among them, compound 1 showed potent inhibitory effect on MMP-1 and the increased type-I procollagen synthesis in ultraviolet B-induced human fibroblast. These results suggest that pedunculagin, an ellagitannin, is a potential candidate for the prevention and treatment of skin aging.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Cell Survival / drug effects
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Cells, Cultured
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Collagen Type I / biosynthesis*
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Down-Regulation / drug effects
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Fibroblasts / drug effects*
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Fibroblasts / enzymology
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Fibroblasts / metabolism
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Fibroblasts / radiation effects*
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Free Radical Scavengers / isolation & purification
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Free Radical Scavengers / pharmacology
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Humans
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Matrix Metalloproteinase 1 / metabolism*
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Matrix Metalloproteinase Inhibitors / isolation & purification
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Matrix Metalloproteinase Inhibitors / pharmacology*
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Molecular Structure
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Pancreatic Elastase / antagonists & inhibitors
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Pancreatic Elastase / metabolism
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Phenols / isolation & purification*
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Phenols / pharmacology*
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Plant Leaves / chemistry
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Quercus / chemistry*
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Skin Aging / drug effects
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Ultraviolet Rays
Substances
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Collagen Type I
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Free Radical Scavengers
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Matrix Metalloproteinase Inhibitors
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Phenols
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Pancreatic Elastase
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Matrix Metalloproteinase 1