The development of a fluorescence polarization immunoassay for aflatoxin detection

Ya Jie Sheng; Sergei Eremin; Tie Jun Mi; Su Xia Zhang; Jian Zhong Shen; Zhan Hui Wang

doi:10.3967/bes2014.027

The development of a fluorescence polarization immunoassay for aflatoxin detection

Biomed Environ Sci. 2014 Feb;27(2):126-9. doi: 10.3967/bes2014.027.

Authors

Ya Jie Sheng¹, Sergei Eremin², Tie Jun Mi¹, Su Xia Zhang¹, Jian Zhong Shen¹, Zhan Hui Wang¹

Affiliations

¹ Department of Veterinary Pharmacology and Toxicology, College of Veterinary Medicine, China Agricultural University, and Beijing Key Laboratory of Detection Technology for Animal-Derived Food Safety, Beijing Laboratory for Food Quality and Safety, Beijing 100193, China.
² Department of Chemistry, M.V. Lomonosov Moscow State University, Moscow 109807, Russia.

PMID: 24625404
DOI: 10.3967/bes2014.027

Abstract

A fluorescence polarization immunoassay (FPIA) was developed for the analysis ofaflatoxins (AFs) using an anti-aflatoxin B1 (AFB1) monoclonal antibody and a novel fluorescein-labeled AFB1 tracer. The FPIA showed an IC50 value of 23.33 ng/mL with a limit of detection of 13.12 ng/mL for AFB1. The cross-reactivities of AFB1, AFB2, AFG1, AFG2, AFM1, and AFM2 with the antibody were 100%, 65.7%, 143%, 23.5%, 111.4%, and 2%, respectively. The group-specificity of anti-AFB1mAb indicated that the FPIA could potentially be used in a screening method for the detection of total AFs, albeit not AFG2 and AFM2. The total time required for analyzing 96 samples in one microplate was less than 5 min. This study demonstrates the potential usefulness of the FPIA as a rapid and simple technique for monitoring AFs.

Publication types

Evaluation Study
Letter
Research Support, Non-U.S. Gov't

MeSH terms

Aflatoxins / analysis*
Fluorescence Polarization Immunoassay

Substances

Aflatoxins