Abstract
Polycomb group (PcG) complexes such as PRC1 mediate transcriptional repression. Here, we show that the plant-specific EMBRYONIC FLOWER1 (EMF1), LIKE HETEROCHROMATIN PROTEIN1, and a histone H3 lysine-4 demethylase form a distinct PcG complex, termed EMF1c, that plays PRC1-like roles and is crucial for regulation of the florigen gene FLOWERING LOCUS T (FT) in Arabidopsis. Long-day photoperiods promote FT expression activation in leaf veins specifically at dusk through the photoperiod pathway to induce Arabidopsis flowering. We found that before dusk and at night, a vascular EMF1c directly represses FT expression to prevent photoperiod-independent flowering, whereas at dusk EMF1 binding to FT chromatin is disrupted by the photoperiod pathway, leading to proper FT activation. Furthermore, a MADS-domain transcription factor and potent floral repressor binds EMF1 to repress FT expression. Our study reveals that the vascular EMF1c integrates inputs from several flowering-regulatory pathways to synchronize flowering time to environmental cues.
Copyright © 2014 Elsevier Inc. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Arabidopsis / genetics
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Arabidopsis / growth & development
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Arabidopsis / metabolism*
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Arabidopsis Proteins / genetics
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Arabidopsis Proteins / metabolism*
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Blotting, Western
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Cell Differentiation
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Cell Proliferation
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Chromatin / genetics
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Chromatin Immunoprecipitation
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Chromosomal Proteins, Non-Histone / genetics
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Chromosomal Proteins, Non-Histone / metabolism
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Florigen / metabolism
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Flowers / growth & development*
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Flowers / metabolism
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Histones / metabolism
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Immunoprecipitation
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Jumonji Domain-Containing Histone Demethylases / genetics
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Jumonji Domain-Containing Histone Demethylases / metabolism
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MADS Domain Proteins / genetics
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MADS Domain Proteins / metabolism
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Photoperiod*
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Plant Leaves / cytology
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Plant Leaves / genetics
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Plant Leaves / metabolism*
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Polycomb-Group Proteins / genetics
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Polycomb-Group Proteins / metabolism*
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RNA, Messenger / genetics
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Real-Time Polymerase Chain Reaction
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Reverse Transcriptase Polymerase Chain Reaction
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Transcriptional Activation
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Two-Hybrid System Techniques
Substances
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Arabidopsis Proteins
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Chromatin
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Chromosomal Proteins, Non-Histone
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EMF1 protein, Arabidopsis
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FLF protein, Arabidopsis
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Florigen
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Histones
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MADS Domain Proteins
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Polycomb-Group Proteins
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RNA, Messenger
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like heterochromatin protein 1
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JMJ14 protein, Arabidopsis
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Jumonji Domain-Containing Histone Demethylases