Fluorescent analog of OSW-1 and its cellular localization

Rika Yamada; Tomoya Takeshita; Masato Hiraizumi; Daisuke Shinohe; Yoshihiro Ohta; Kaori Sakurai

doi:10.1016/j.bmcl.2014.02.009

Fluorescent analog of OSW-1 and its cellular localization

Bioorg Med Chem Lett. 2014 Apr 1;24(7):1839-42. doi: 10.1016/j.bmcl.2014.02.009. Epub 2014 Feb 14.

Authors

Rika Yamada¹, Tomoya Takeshita¹, Masato Hiraizumi¹, Daisuke Shinohe¹, Yoshihiro Ohta¹, Kaori Sakurai²

Affiliations

¹ Tokyo University of Agriculture and Technology, School of Engineering, Koganei-shi, Tokyo 184-8588, Japan.
² Tokyo University of Agriculture and Technology, School of Engineering, Koganei-shi, Tokyo 184-8588, Japan. Electronic address: sakuraik@cc.tuat.ac.jp.

PMID: 24613377
DOI: 10.1016/j.bmcl.2014.02.009

Abstract

OSW-1 is a steroidal saponin, which has emerged as an attractive anticancer agent with highly cancer cell selective activity. A fluorescent analog was prepared from the natural product to analyze its cellular uptake and localization. We found that the fluorescent analog is rapidly internalized into cells and is primarily distributed in endoplasmic reticulum and Golgi apparatus.

Keywords: Anticancer agent; Cellular localization; Cellular uptake; Fluorescent probe; Steroidal glycoside.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cholestenones / chemistry
Cholestenones / pharmacokinetics*
Fluorescence
Fluorescent Dyes / chemistry
Fluorescent Dyes / pharmacokinetics*
HeLa Cells
Humans
Microscopy, Fluorescence
Molecular Conformation
Saponins / chemistry
Saponins / pharmacokinetics*
Temperature
Tissue Distribution

Substances

Cholestenones
Fluorescent Dyes
Saponins
OSW 1