Two-dimensional gel electrophoresis image registration using block-matching techniques and deformation models

Alvaro Rodriguez; Carlos Fernandez-Lozano; Julian Dorado; Juan R Rabuñal

doi:10.1016/j.ab.2014.02.027

Two-dimensional gel electrophoresis image registration using block-matching techniques and deformation models

Anal Biochem. 2014 Jun 1:454:53-9. doi: 10.1016/j.ab.2014.02.027. Epub 2014 Mar 5.

Authors

Alvaro Rodriguez¹, Carlos Fernandez-Lozano², Julian Dorado², Juan R Rabuñal³

Affiliations

¹ Department of Information and Communications Technologies, University of A Coruña, Campus de Elviña, 15071 A Coruña, Spain. Electronic address: arodriguezta@udc.es.
² Department of Information and Communications Technologies, University of A Coruña, Campus de Elviña, 15071 A Coruña, Spain.
³ Department of Information and Communications Technologies, University of A Coruña, Campus de Elviña, 15071 A Coruña, Spain; Centre of Technological Innovation in Construction and Civil Engineering (CITEEC), University of A Coruña, Campus de Elviña, 15071 A Coruña, Spain.

PMID: 24613260
DOI: 10.1016/j.ab.2014.02.027

Abstract

Block-matching techniques have been widely used in the task of estimating displacement in medical images, and they represent the best approach in scenes with deformable structures such as tissues, fluids, and gels. In this article, a new iterative block-matching technique-based on successive deformation, search, fitting, filtering, and interpolation stages-is proposed to measure elastic displacements in two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) images. The proposed technique uses different deformation models in the task of correlating proteins in real 2D electrophoresis gel images, obtaining an accuracy of 96.6% and improving the results obtained with other techniques. This technique represents a general solution, being easy to adapt to different 2D deformable cases and providing an experimental reference for block-matching algorithms.

Keywords: 2D gel images; Block-matching; Deformations; Electrophoresis; Optical flow; Proteomics.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Algorithms
Electrophoresis, Gel, Two-Dimensional / methods*
Image Processing, Computer-Assisted / methods*
Models, Theoretical*
Proteomics / methods*