Femtomolar level detection of BRCA1 gene using a gold nanoparticle labeled sandwich type DNA sensor

Colloids Surf B Biointerfaces. 2014 May 1:117:7-13. doi: 10.1016/j.colsurfb.2014.02.009. Epub 2014 Feb 15.

Abstract

We demonstrate the amplified detection of BRCAI gene based on the gold nanoparticle labeled DNA sensor. The sensor was based on a "sandwich" detection strategy, which involved an immobilized capture probe DNA (DNA-c), Target DNA (DNA-t) and gold nanoparticle conjugated reporter probe DNA (DNA-r.AuNP). The sensor surface was characterized by scanning electron microscopy (SEM) and scanning tunneling microscopy (STM). Detection capability of the sensor was studied with I-V measurements using either scanning tunneling microscopy (STM) or Keithley 2400 Source Meter SMU Instrument. The DNA sensor could detect up to 1 fM DNA target (5.896 fg of BRCA 1 gene/ml) and exhibited excellent selectivity against noncomplementary sequences and three base mismatch complementary targets. Good reproducibility, high sensitivity, good stability and reusability of the developed sensor surface showed its application in early cancer diagnosis.

Keywords: BRCA 1 gene; DNA sensor; Femtomolar detection; Gold nanoparticles.; STM I–V characteristics.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biosensing Techniques / methods*
  • DNA Probes / chemistry*
  • Electrochemical Techniques
  • Genes, BRCA1*
  • Gold / chemistry*
  • Humans
  • Metal Nanoparticles / chemistry*
  • Metal Nanoparticles / ultrastructure
  • Nucleic Acid Hybridization
  • Quartz Crystal Microbalance Techniques

Substances

  • DNA Probes
  • Gold