A combined proteomic and transcriptomic analysis on sulfur metabolism pathways of Arabidopsis thaliana under simulated acid rain

Tingwu Liu; Juan A Chen; Wenhua Wang; Martin Simon; Feihua Wu; Wenjun Hu; Juan B Chen; Hailei Zheng

doi:10.1371/journal.pone.0090120

A combined proteomic and transcriptomic analysis on sulfur metabolism pathways of Arabidopsis thaliana under simulated acid rain

PLoS One. 2014 Mar 3;9(3):e90120. doi: 10.1371/journal.pone.0090120. eCollection 2014.

Authors

Tingwu Liu¹, Juan A Chen², Wenhua Wang³, Martin Simon², Feihua Wu², Wenjun Hu², Juan B Chen², Hailei Zheng⁴

Affiliations

¹ Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystems, College of the Environment and Ecology, Xiamen University, Xiamen, Fujian, P. R. China; Department of Biology, Huaiyin Normal University, Huaian, Jiangsu, P. R. China.
² Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystems, College of the Environment and Ecology, Xiamen University, Xiamen, Fujian, P. R. China.
³ Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystems, College of the Environment and Ecology, Xiamen University, Xiamen, Fujian, P. R. China; Department of Biology, Duke University, Durham, North Carolina, United States of America.
⁴ Key Laboratory of the Ministry of Education for Coastal and Wetland Ecosystems, College of the Environment and Ecology, Xiamen University, Xiamen, Fujian, P. R. China; State Key Laboratory of Marine Environmental Science, Xiamen University, Xiamen, Fujian, P. R. China.

Abstract

With rapid economic development, most regions in southern China have suffered acid rain (AR) pollution. In our study, we analyzed the changes in sulfur metabolism in Arabidopsis under simulated AR stress which provide one of the first case studies, in which the systematic responses in sulfur metabolism were characterized by high-throughput methods at different levels including proteomic, genomic and physiological approaches. Generally, we found that all of the processes related to sulfur metabolism responded to AR stress, including sulfur uptake, activation and also synthesis of sulfur-containing amino acid and other secondary metabolites. Finally, we provided a catalogue of the detected sulfur metabolic changes and reconstructed the coordinating network of their mutual influences. This study can help us to understand the mechanisms of plants to adapt to AR stress.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Acid Rain*
Arabidopsis / genetics
Arabidopsis / metabolism*
Arabidopsis Proteins / genetics
Arabidopsis Proteins / metabolism*
Electrophoresis, Gel, Two-Dimensional
Electrophoresis, Polyacrylamide Gel
Glutathione / metabolism
Proteomics*
RNA, Plant / metabolism*
Reactive Oxygen Species / metabolism
Real-Time Polymerase Chain Reaction
Sulfur / metabolism*
Transcriptome*

Substances

Acid Rain
Arabidopsis Proteins
RNA, Plant
Reactive Oxygen Species
Sulfur
Glutathione

Grants and funding

This study was financially supported by the Natural Science Foundation of China (NSFC) (30930076, 31260057, 30770192 and 30670317), the Scholarship Award for Excellent Doctoral Student granted by Ministry of Education, the Foundation of the Chinese Ministry of Education (20070384033, 209084), the Program for New Century Excellent Talents in Xiamen University (NCETXMU X07115) and a Changjiang Scholarship (X09111). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.