Antioxidant effects of whey protein on muscle C2C12 cells

Efthalia Kerasioti; Dimitrios Stagos; Alexandros Priftis; Stefanos Aivazidis; Aristidis M Tsatsakis; A Wallace Hayes; Demetrios Kouretas

doi:10.1016/j.foodchem.2014.01.066

Antioxidant effects of whey protein on muscle C2C12 cells

Food Chem. 2014 Jul 15:155:271-8. doi: 10.1016/j.foodchem.2014.01.066. Epub 2014 Jan 30.

Authors

Efthalia Kerasioti¹, Dimitrios Stagos², Alexandros Priftis³, Stefanos Aivazidis⁴, Aristidis M Tsatsakis⁵, A Wallace Hayes⁶, Demetrios Kouretas⁷

Affiliations

¹ Department of Biochemistry and Biotechnology, University of Thessaly, Ploutonos 26 & Aiolou St., Larissa 41221, Greece. Electronic address: e-f-thalia@hotmail.com.
² Department of Biochemistry and Biotechnology, University of Thessaly, Ploutonos 26 & Aiolou St., Larissa 41221, Greece. Electronic address: stagkos@med.uth.gr.
³ Department of Biochemistry and Biotechnology, University of Thessaly, Ploutonos 26 & Aiolou St., Larissa 41221, Greece. Electronic address: alexandros.priftis@hotmail.com.
⁴ Department of Biochemistry and Biotechnology, University of Thessaly, Ploutonos 26 & Aiolou St., Larissa 41221, Greece. Electronic address: asnf19@hotmail.com.
⁵ Department of Forensic and Toxicology, Medical School, University of Crete, Heraklion 71409, Greece. Electronic address: aris@med.uoc.gr.
⁶ Harvard School of Public Health, 677 Huntington Avenue, Boston, MA 02115, USA; Spherix Consulting, Inc., 6430 Rockledge Drive #503, Bethesda, MD 20817, USA. Electronic address: awhayes@spherix.com.
⁷ Department of Biochemistry and Biotechnology, University of Thessaly, Ploutonos 26 & Aiolou St., Larissa 41221, Greece. Electronic address: dkouret@uth.gr.

PMID: 24594185
DOI: 10.1016/j.foodchem.2014.01.066

Abstract

In the present study, the in vitro scavenging activity of sheep whey protein against free radicals, as well as its reducing power were determined and compared with that of beef protein, soy protein and cow whey protein. Moreover, the possible protective effects of sheep whey protein from tert-butyl hydroperoxide (tBHP)-induced oxidative stress in muscle C2C12 cells were determined by assessing oxidative stress markers by flow cytometry and spectrophotometry. The results showed that sheep whey protein scavenged DPPH, ABTS(+) and OH radicals with IC50 values of 3.1, 4.1 and 1.8 mg of protein/ml. Moreover, the reducing power activity assessed with potassium ferricyanide of sheep whey protein was 1.3mg/ml. As regards to the antioxidant effects in muscle cell line, sheep whey protein at 0.78, 1.56, 3.12 and 6.24 mg of protein/ml increased GSH levels up to 138%, lowered TBARS levels up to 25% and decreased ROS levels up to 41.4%.

Keywords: C2C12 muscle cells; GSH; Hydroxyl radical; ROS; Reducing power; TBARS; Whey protein.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cell Line
Glutathione / metabolism
Lipid Peroxidation
Mice
Milk Proteins / metabolism*
Muscle Cells / metabolism*
Oxidative Stress
Reactive Oxygen Species / metabolism
Sheep
Whey Proteins

Substances

Milk Proteins
Reactive Oxygen Species
Whey Proteins
Glutathione