Simultaneous production of fatty acid methyl esters and diglycerides by four recombinant Candida rugosa lipase's isozymes

Shu-Wei Chang; Myron Huang; Yu-Hsun Hsieh; Ying-Ting Luo; Tsung-Ta Wu; Chia-Wen Tsai; Chin-Shuh Chen; Jei-Fu Shaw

doi:10.1016/j.foodchem.2014.01.035

Simultaneous production of fatty acid methyl esters and diglycerides by four recombinant Candida rugosa lipase's isozymes

Food Chem. 2014 Jul 15:155:140-5. doi: 10.1016/j.foodchem.2014.01.035. Epub 2014 Jan 23.

Authors

Shu-Wei Chang¹, Myron Huang², Yu-Hsun Hsieh³, Ying-Ting Luo⁴, Tsung-Ta Wu⁵, Chia-Wen Tsai⁶, Chin-Shuh Chen², Jei-Fu Shaw⁷

Affiliations

¹ Department of Medicinal Botanicals and Health Applications, Dayeh University, Changhua 515, Taiwan. Electronic address: swchang@mail.dyu.edu.tw.
² Department of Food Science and Biotechnology, National Chung Hsing University, Taichung 402, Taiwan.
³ Department of Life Science, National Chung Hsing University, Taichung 402, Taiwan.
⁴ Department of Bioindustry Technology, Dayeh University, Changhua 515, Taiwan.
⁵ Department of Chemical Engineering, National Chung Hsing University, Taichung 402, Taiwan.
⁶ Department of Nutrition, China Medical University, No. 91, Hsueh-Shih Rd., Taichung 404, Taiwan.
⁷ Department of Biological Science & Technology, I-Shou University, Kaohsiung City 84001, Taiwan; Department of Food Science and Biotechnology, National Chung Hsing University, Taichung 402, Taiwan; Department of Life Science, National Chung Hsing University, Taichung 402, Taiwan; Agricultural Biotechnology Research Center, Academia Sinica, Nankang, Taipei 11529, Taiwan; Agricultural Biotechnology Center, National Chung Hsing University, Taichung 402, Taiwan. Electronic address: shawjf@isu.edu.tw.

PMID: 24594166
DOI: 10.1016/j.foodchem.2014.01.035

Abstract

In this study, the catalytic efficiency of four recombinant CRL (Candida rugosa lipase) isozymes (LIP1-LIP4) towards the production of fatty acid methyl ester (FAME) was compared and evaluated as an alternative green method for industrial applications. The results indicated that the recombinant C. rugosa LIP1 enzyme exhibited the highest catalytic efficiency for FAME production compared to the recombinant C. rugosa LIP2-LIP4 enzymes. The optimal conditions were as follows: pH 7.0, methanol/soybean oil molar ratio: 3/1, enzyme amount: 2U (1.6 μL), reaction temperature: 20°C, 22 h of reaction time, and 3 times of methanol addition (1 mol/6h), and resulted in 61.5 ± 1.5 wt.% of FAME conversion. The reaction product contained also 10 wt.% of DAG with a ratio of 1,3-DAG to 1,2-DAG of approximately 4:6, and can be potentially used in industrial applications as a food emulsifier.

Keywords: Candida rugosa lipase; Diacylglycerol; Emulsifier; Esterification; Fatty acid methyl ester; Isozymes.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Biocatalysis
Candida / enzymology*
Diglycerides / chemistry*
Esters / chemistry*
Fatty Acids / chemistry*
Fungal Proteins / chemistry*
Fungal Proteins / genetics
Fungal Proteins / metabolism
Isoenzymes / antagonists & inhibitors
Isoenzymes / chemistry
Isoenzymes / genetics
Lipase / chemistry
Lipase / genetics
Lipase / metabolism
Recombinant Proteins / chemistry
Recombinant Proteins / genetics
Recombinant Proteins / metabolism
Substrate Specificity

Substances

Diglycerides
Esters
Fatty Acids
Fungal Proteins
Isoenzymes
Recombinant Proteins
Lipase