High genetic stability of peroral infection factors from Anticarsia gemmatalis MNPV over 20years of sampling

Briana C Ferreira; Fernando L Melo; Marlinda L Souza; Flávio Moscardi; Sônia N Báo; Bergmann M Ribeiro

doi:10.1016/j.jip.2014.02.007

High genetic stability of peroral infection factors from Anticarsia gemmatalis MNPV over 20years of sampling

J Invertebr Pathol. 2014 May:118:66-70. doi: 10.1016/j.jip.2014.02.007. Epub 2014 Feb 28.

Authors

Briana C Ferreira¹, Fernando L Melo², Marlinda L Souza³, Flávio Moscardi⁴, Sônia N Báo², Bergmann M Ribeiro⁵

Affiliations

¹ Programa de Pós-Graduação em Patologia Molecular, Faculdade de Medicina, Campus Darcy Ribeiro, Universidade de Brasília (UnB), Asa Norte, 70910-900 Brasília, DF, Brazil; Embrapa Recursos Genéticos e Biotecnologia (Cenargen), Parque Estação Biológica, W5 Norte Final, 70770-917 Brasília, DF, Brazil.
² Programa de Pós-Graduação em Patologia Molecular, Faculdade de Medicina, Campus Darcy Ribeiro, Universidade de Brasília (UnB), Asa Norte, 70910-900 Brasília, DF, Brazil.
³ Embrapa Recursos Genéticos e Biotecnologia (Cenargen), Parque Estação Biológica, W5 Norte Final, 70770-917 Brasília, DF, Brazil.
⁴ Centro de Ciências Agrárias, Universidade Estadual de Londrina (UEL), 86051-990 Londrina, PR, Brazil.
⁵ Programa de Pós-Graduação em Patologia Molecular, Faculdade de Medicina, Campus Darcy Ribeiro, Universidade de Brasília (UnB), Asa Norte, 70910-900 Brasília, DF, Brazil. Electronic address: bergmann@unb.br.

PMID: 24590109
DOI: 10.1016/j.jip.2014.02.007

Abstract

The Anticarsia gemmatalis multiple nucleopolyhedrovirus (AgMNPV) has been used as a biopesticide since the early 1980s in Brazil to control the major pest of soybean crops, the velvetbean caterpillar, Anticarsia gemmatalis. To monitor the genetic diversity over space and time we sequenced four pif genes (pif1, pif2, pif3 and pif4) from AgMNPV isolates collected from different regions of South America, as well as of seasonal isolates, sampled during a two-decade field experiment. Although all genes presented low levels of polymorphism, the pif-2 carries a slightly higher number of polymorphic sites. Overall, this study reveals that pif genes have remained stable after 20 years of repeated field application.

Keywords: Baculovirus Anticarsia gemmatalis MNPV; Geographical isolates; Pif genes; Seasonal isolates; Stability.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Genes, Viral / genetics*
Nucleopolyhedroviruses / genetics*
Pest Control, Biological
Polymorphism, Single Nucleotide
Reverse Transcriptase Polymerase Chain Reaction
Virulence Factors / genetics*

Substances

Virulence Factors