Identification of dermatophyte species using genomic in situ hybridization (GISH)

J Microbiol Methods. 2014 May:100:32-41. doi: 10.1016/j.mimet.2014.02.012. Epub 2014 Feb 28.

Abstract

A genomic in situ hybridization (GISH)-based method for dermatophyte identification has been developed. Using specific GISH probes, discrimination between Trichophyton interdigitale, Trichophyton rubrum and Microsporum canis has been conducted. Moreover, GISH has been found particularly helpful when proper dermatophyte identification was difficult due to ambiguous PCR-RFLP patterns.

Keywords: Dermatophytes; GISH; Microsporum; PCR–RFLP; Trichophyton.

Publication types

  • Evaluation Study

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Aged, 80 and over
  • Animals
  • Arthrodermataceae / classification
  • Arthrodermataceae / genetics*
  • Arthrodermataceae / isolation & purification*
  • Child
  • Child, Preschool
  • Dermatomycoses / diagnosis*
  • Dermatomycoses / microbiology
  • Female
  • Genome, Fungal
  • Humans
  • In Situ Hybridization / methods*
  • Male
  • Microsporum / classification
  • Microsporum / genetics
  • Microsporum / isolation & purification
  • Middle Aged
  • Trichophyton / classification
  • Trichophyton / genetics
  • Trichophyton / isolation & purification
  • Young Adult