Biodegradation of neonicotinoid insecticide, imidacloprid by restriction enzyme mediated integration (REMI) generated Trichoderma mutants

Chemosphere. 2014 Oct:112:526-30. doi: 10.1016/j.chemosphere.2014.01.023. Epub 2014 Feb 28.

Abstract

REMI (restriction enzyme-mediated integration) technique was employed to construct Trichoderma atroviride strain T23 mutants with degrading capability of neonicotinoid insecticide, imidacloprid. The plasmid pBluescript II KS-hph used for integration in REMI mutants was confirmed by PCR and Southern hybridization. Among 153 transformants, 57% of them have showed higher neonicotinoid insecticide, imidacloprid, degradation ability than the wild strain T23 (p<0.01). More specifically, seven single-copied T. atroviride T23 transformants have confirmed a 30% higher degradation rate than their parent isolate. Among all transformed mutants, a 95% imidacloprid degradation rate was identified as the highest. This study, thus, provided an effective approach for improving neonicotinoid insecticide-degrading capability using REMI transformed T. atroviride mutants.

Keywords: Biodegradation; Imidacloprid; Neonicotinoid insecticide; REMI; Trichoderma.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biodegradation, Environmental
  • DNA Restriction Enzymes / metabolism*
  • Environmental Pollutants / isolation & purification
  • Environmental Pollutants / metabolism*
  • Genetic Engineering*
  • Imidazoles / isolation & purification
  • Imidazoles / metabolism*
  • Insecticides / isolation & purification
  • Insecticides / metabolism*
  • Neonicotinoids
  • Nitro Compounds / isolation & purification
  • Nitro Compounds / metabolism*
  • Plasmids / genetics
  • Trichoderma / genetics*
  • Trichoderma / metabolism*

Substances

  • Environmental Pollutants
  • Imidazoles
  • Insecticides
  • Neonicotinoids
  • Nitro Compounds
  • imidacloprid
  • DNA Restriction Enzymes