Quantitative field testing Heterodera glycines from metagenomic DNA samples isolated directly from soil under agronomic production

Yan Li; Gary W Lawrence; Shien Lu; Clarissa Balbalian; Vincent P Klink

doi:10.1371/journal.pone.0089887

Quantitative field testing Heterodera glycines from metagenomic DNA samples isolated directly from soil under agronomic production

PLoS One. 2014 Feb 24;9(2):e89887. doi: 10.1371/journal.pone.0089887. eCollection 2014.

Authors

Yan Li¹, Gary W Lawrence¹, Shien Lu¹, Clarissa Balbalian¹, Vincent P Klink²

Affiliations

¹ Department of Biochemistry, Molecular Biology, Entomology and Plant Pathology, Mississippi State University, Mississippi State, Mississippi, United States of America.
² Department of Biological Sciences, Mississippi State University, Mississippi State, Mississippi, United States of America.

Abstract

A quantitative PCR procedure targeting the Heterodera glycines ortholog of the Caenorhabditis elegans uncoordinated-78 gene was developed. The procedure estimated the quantity of H. glycines from metagenomic DNA samples isolated directly from field soil under agronomic production. The estimation of H. glycines quantity was determined in soil samples having other soil dwelling plant parasitic nematodes including Hoplolaimus, predatory nematodes including Mononchus, free-living nematodes and biomass. The methodology provides a framework for molecular diagnostics of nematodes from metagenomic DNA isolated directly from field soil.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Base Sequence
Caenorhabditis elegans Proteins / genetics
DNA Primers / genetics
Metagenome / genetics*
Microfilament Proteins / genetics
Molecular Sequence Data
Polymerase Chain Reaction
Sequence Analysis, DNA
Soil / chemistry*
Soil / parasitology*
Species Specificity
Tylenchoidea / genetics*

Substances

Caenorhabditis elegans Proteins
DNA Primers
Microfilament Proteins
Soil
unc-78 protein, C elegans

Grants and funding

The work was supported by the Mississippi Soybean Promotion Board. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.