Pharmacokinetic comparison of seven major bio-active components in normal and blood deficiency rats after oral administration of Danggui Buxue decoction by UPLC-TQ/MS

Xuqin Shi; Yuping Tang; Huaxu Zhu; Weixia Li; Wei Li; Zhenhao Li; Niancui Luo; Jin-ao Duan

doi:10.1016/j.jep.2014.02.004

Pharmacokinetic comparison of seven major bio-active components in normal and blood deficiency rats after oral administration of Danggui Buxue decoction by UPLC-TQ/MS

J Ethnopharmacol. 2014 Apr 11;153(1):169-77. doi: 10.1016/j.jep.2014.02.004. Epub 2014 Feb 26.

Authors

Xuqin Shi¹, Yuping Tang², Huaxu Zhu¹, Weixia Li¹, Wei Li¹, Zhenhao Li¹, Niancui Luo¹, Jin-ao Duan¹

Affiliations

¹ Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, National and Local Collaborative Engeering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, Nanjing University of Chinese Medicine, Nanjing 210023, China.
² Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, National and Local Collaborative Engeering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine, Nanjing University of Chinese Medicine, Nanjing 210023, China. Electronic address: yupingtang@njutcm.edu.cn.

PMID: 24583239
DOI: 10.1016/j.jep.2014.02.004

Abstract

Ethnopharmacological relevance: Blood deficiency is commonly encountered among women, and is the root of many gynecological disorders. Danggui Buxue Decoction (DBD), a classical traditional Chinese formula which is composed of Astragali Radix (AR) and Angelicae Sinensis Radix (ASR) at the ratio of 5:1 (w/w), is widely used in TCM clinics for treatment of blood deficiency syndrome. This study is to compare the in vivo pharmacokinetic properties of seven major bio-active components in normal and blood deficiency rats after oral administration of DBD.

Materials and methods: Blood deficiency rats were induced by bleeding from orbit at the dosages of 5.0mL/kg each day for 12 days. Normal and blood deficiency rats were administrated of DBD on the 12th day at the dosage of 20g/kg, and blood was collected at different time points after then. Concentrations of ferulic acid, caffeic acid, butylphthalide, ligustilide, calycosin-7-O-β-glucoside, ononin, and astragaloside IV in plasma were quantified by UPLC-TQ/MS, and the main pharmacokinetic parameters were calculated by DAS 2.0.

Results: It was found that Cmax, Tmax and MRT0~T of astragaloside IV, Cmax, T1/2Z, AUC0~T and MRT0~T of calycosin-7-O-β-glucoside, T1/2Z and AUC0~T of ferulic acid, T1/2Z, AUC0~T and MRT0~T of ononin, and MRT0~T of ligustilide, butylphthalide, and caffeic acid in blood deficiency rats was significantly different (P<0.05) from normal rats.

Conclusions: This study was the first report about pharmacokinetic investigation in blood deficiency animals which was conducted by bleeding. And the results demonstrated that the seven DBD constituents in normal and blood deficiency rats had obvious differences in some pharmacokinetic characteristics, suggesting that the rate and extent of drug metabolism were altered in blood deficiency animals.

Keywords: Astragaloside IV (PubChem CID: 13943297); Bleeding; Blood deficiency; Butylphthalide (PubChem CID: 61361); Caffeic acid (PubChem CID: 689043); Calycosin-7-O-β-glucoside ( PubChem CID: 44257232); Comparison; Danggui Buxue Decoction; Ferulic acid (PubChem CID: 445858); Ligustilide (PubChem CID: 5319022); Ononin (PubChem CID: 442813); Pharmacokinetic; UPLC-TQ/MS.

Publication types

Research Support, Non-U.S. Gov't
Validation Study

MeSH terms

Animals
Area Under Curve
Chromatography, High Pressure Liquid / methods*
Disease Models, Animal
Drugs, Chinese Herbal / pharmacokinetics*
Female
Hematologic Diseases / metabolism*
Male
Mass Spectrometry / methods*
Medicine, Chinese Traditional
Rats
Rats, Sprague-Dawley

Substances

Drugs, Chinese Herbal
danggui buxue decoction