Freeze-drying of ovalbumin loaded mesoporous silica nanoparticle vaccine formulation increases antigen stability under ambient conditions

Karishma T Mody; Donna Mahony; Antonino S Cavallaro; Frances Stahr; Shi Zhang Qiao; Timothy J Mahony; Neena Mitter

doi:10.1016/j.ijpharm.2014.01.037

Freeze-drying of ovalbumin loaded mesoporous silica nanoparticle vaccine formulation increases antigen stability under ambient conditions

Int J Pharm. 2014 Apr 25;465(1-2):325-32. doi: 10.1016/j.ijpharm.2014.01.037. Epub 2014 Feb 26.

Authors

Karishma T Mody¹, Donna Mahony², Antonino S Cavallaro³, Frances Stahr⁴, Shi Zhang Qiao⁵, Timothy J Mahony⁶, Neena Mitter⁷

Affiliations

¹ Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, Queensland, Australia. Electronic address: k.mody@uq.edu.au.
² Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, Queensland, Australia. Electronic address: d.mahony1@uq.edu.au.
³ Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, Queensland, Australia. Electronic address: a.cavallaro@uq.edu.au.
⁴ Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, Queensland, Australia. Electronic address: s4075611@student.uq.edu.au.
⁵ Australian Institute for Bioengineering and Nanotechnology, The University of Queensland, Queensland, Australia. Electronic address: s.qiao@adelaide.edu.au.
⁶ Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, Queensland, Australia. Electronic address: t.mahony@uq.edu.au.
⁷ Queensland Alliance for Agriculture and Food Innovation, The University of Queensland, Queensland, Australia. Electronic address: n.mitter@uq.edu.au.

PMID: 24583208
DOI: 10.1016/j.ijpharm.2014.01.037

Abstract

Amino functionalised mesoporous silica nanoparticles (AM-41) have been identified as a promising vaccine delivery material. The capacity of AM-41 to stabilise vaccine components at ambient temperature (23-27°C) was determined by adsorbing the model antigen ovalbumin (OVA) to AM-41 particles (OVA-41). The OVA-41 was successfully freeze-dried using the excipients 5% trehalose and 1% PEG8000. The immunological activity of OVA and the nanoparticle structure were maintained following two months storage at ambient temperature. The results of immunisation studies in mice with reconstituted OVA-41 demonstrated the induction of humoral and cell-meditated immune responses. The capacity of AM-41 particles to facilitate ambient storage of vaccine components without the loss of immunological potency will underpin the further development of this promising vaccine delivery platform.

Keywords: Excipients; Freeze-drying; Mesoporous silica nanoparticles; Protein; Stability; Vaccine delivery.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Antigens / administration & dosage
Antigens / chemistry*
Antigens / immunology
Cattle
Cell Line
Cell Survival / drug effects
Chemistry, Pharmaceutical
Drug Carriers*
Drug Stability
Drug Storage
Excipients / chemistry
Freeze Drying*
Immunity, Cellular / drug effects
Immunity, Humoral / drug effects
Immunization
Injections, Subcutaneous
Mice, Inbred C57BL
Nanoparticles*
Nanotechnology
Ovalbumin / administration & dosage
Ovalbumin / chemistry*
Ovalbumin / immunology
Polyethylene Glycols / chemistry
Porosity
Protein Stability
Silicon Dioxide / administration & dosage
Silicon Dioxide / chemistry*
Silicon Dioxide / toxicity
Technology, Pharmaceutical / methods*
Temperature
Trehalose / chemistry
Vaccines / administration & dosage
Vaccines / chemistry*
Vaccines / immunology

Substances

Antigens
Drug Carriers
Excipients
Vaccines
Polyethylene Glycols
Silicon Dioxide
Ovalbumin
Trehalose